GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:28262819

From GONUTS
Jump to: navigation, search
Citation

Su, C, Zhao, H, Zhao, Y, Ji, H, Wang, Y, Zhi, L and Li, X (2017) RUG3 and ATM synergistically regulate the alternative splicing of mitochondrial nad2 and the DNA damage response in Arabidopsis thaliana. Sci Rep 7:43897

Abstract

The root apical meristem (RAM) determines both RAM activity and the growth of roots. Plant roots are constantly exposed to adverse environmental stresses that can cause DNA damage or cell cycle arrest in the RAM; however, the mechanism linking root meristematic activity and RAM size to the DNA damage response (DDR) is unclear. Here, we demonstrate that a loss of function in RCC1/UVR8/GEF-Like 3 (RUG3) substantially augmented the DDR and produced a cell cycle arrest in the RAM in rug3 mutant, leading to root growth retardation. Furthermore, the mutation of RUG3 caused increased intracellular reactive oxygen species (ROS) levels, and ROS scavengers improved the observed cell cycle arrest and reduced RAM activity level in rug3 plants. Most importantly, we detected a physical interaction between RUG3 and ataxia telangiectasia mutated (ATM), a key regulator of the DDR, suggesting that they synergistically modulated the alternative splicing of nad2. Our findings reveal a novel synergistic effect of RUG3 and ATM on the regulation of mitochondrial function, redox homeostasis, and the DDR in the RAM, and outline a protective mechanism for DNA damage repair and the restoration of mitochondrial function that involves RUG3-mediated mitochondrial retrograde signaling and the activation of an ATM-mediated DDR pathway.

Links

PubMed PMC5338318 Online version:10.1038/srep43897

Keywords

Alternative Splicing; Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Ataxia Telangiectasia Mutated Proteins/genetics; Ataxia Telangiectasia Mutated Proteins/metabolism; DNA Damage; DNA Repair; Electron Transport Complex I/genetics; Electron Transport Complex I/metabolism; Gene Expression Regulation, Plant; Meristem/enzymology; Meristem/physiology; Mitochondrial Proteins/genetics; Mitochondrial Proteins/metabolism; Oxidation-Reduction; Protein Binding; Protein Interaction Mapping; Stress, Physiological

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:RUG3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9M3G7

F

Seeded From UniProt

complete

ARATH:RUG3

involved_in

GO:0008380: RNA splicing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RUG3

involved_in

GO:0010228: vegetative to reproductive phase transition of meristem

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RUG3

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:RUG3

involved_in

GO:2001020: regulation of response to DNA damage stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RUG3

involved_in

GO:0072702: response to methyl methanesulfonate

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATM

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9FJG9

F

Seeded From UniProt

complete

ARATH:ATM

involved_in

GO:0008380: RNA splicing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ATM

involved_in

GO:2001020: regulation of response to DNA damage stimulus

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.