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PMID:28221134
| Citation |
Capitanio, JS, Montpetit, B and Wozniak, RW (2017) Human Nup98 regulates the localization and activity of DExH/D-box helicase DHX9. Elife 6 |
|---|---|
| Abstract |
Beyond their role at nuclear pore complexes, some nucleoporins function in the nucleoplasm. One such nucleoporin, Nup98, binds chromatin and regulates gene expression. To gain insight into how Nup98 contributes to this process, we focused on identifying novel binding partners and understanding the significance of these interactions. Here we report on the identification of the DExH/D-box helicase DHX9 as an intranuclear Nup98 binding partner. Various results, including in vitro assays, show that the FG/GLFG region of Nup98 binds to N- and C-terminal regions of DHX9 in an RNA facilitated manner. Importantly, binding of Nup98 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated transcription. Consistent with these observations, our analysis revealed that Nup98 and DHX9 bind interdependently to similar gene loci and their transcripts. Based on our results, we propose that Nup98 functions as a co-factor that regulates DHX9 and, potentially, other RNA helicases. |
| Links |
PubMed PMC5338925 Online version:10.7554/eLife.18825 |
| Keywords |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
located_in |
GO:0005635: nuclear envelope |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0016604: nuclear body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q08211 |
F |
Seeded From UniProt |
complete | ||
|
located_in |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003724: RNA helicase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
located_in |
GO:0016604: nuclear body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P52948 |
F |
Seeded From UniProt |
complete | ||
| GO:0005654: nucleoplasm |
ECO:0000314: |
C |
Human nup98. Figure 3, immunofluorescence spectroscopy confirmed localization to the nucleoplasm. |
complete | ||||
|
part_of |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016604: nuclear body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003729: mRNA binding |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:1990904: ribonucleoprotein complex |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:1990841: promoter-specific chromatin binding |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0048026: positive regulation of mRNA splicing, via spliceosome |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005635: nuclear envelope |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003713: transcription coactivator activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0005654: nucleoplasm |
ECO:0000314: |
C |
Human DHX9. Figure 3, immunofluorescence spectroscopy shows localization to the nucleoplasm. |
complete | ||||
|
part_of |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016604: nuclear body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003729: mRNA binding |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004004: ATP-dependent RNA helicase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:1990904: ribonucleoprotein complex |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:1990841: promoter-specific chromatin binding |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000380: alternative mRNA splicing, via spliceosome |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
| GO:0005654: nucleoplasm |
ECO:0000314: |
C |
Human hnRNP U. Figure 3, immunofluorescence spectroscopy confirmed localization to the nucleoplasm. |
complete | ||||
|
part_of |
GO:0005654: nucleoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
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