GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:27836698

From GONUTS
Jump to: navigation, search
Citation

Vozza, A, De Leonardis, F, Paradies, E, De Grassi, A, Pierri, CL, Parisi, G, Marobbio, CMT, Lasorsa, FM, Muto, L, Capobianco, L, Dolce, V, Raho, S and Fiermonte, G (2017) Biochemical characterization of a new mitochondrial transporter of dephosphocoenzyme A in Drosophila melanogaster. Biochim Biophys Acta Bioenerg 1858:137-146

Abstract

CoA is an essential cofactor that holds a central role in cell metabolism. Although its biosynthetic pathway is conserved across the three domains of life, the subcellular localization of the eukaryotic biosynthetic enzymes and the mechanism behind the cytosolic and mitochondrial CoA pools compartmentalization are still under debate. In humans, the transport of CoA across the inner mitochondrial membrane has been ascribed to two related genes, SLC25A16 and SLC25A42 whereas in D. melanogaster genome only one gene is present, CG4241, phylogenetically closer to SLC25A42. CG4241 encodes two alternatively spliced isoforms, dPCoAC-A and dPCoAC-B. Both isoforms were expressed in Escherichia coli, but only dPCoAC-A was successfully reconstituted into liposomes, where transported dPCoA and, to a lesser extent, ADP and dADP but not CoA, which was a powerful competitive inhibitor. The expression of both isoforms in a Saccharomyces cerevisiae strain lacking the endogenous putative mitochondrial CoA carrier restored the growth on respiratory carbon sources and the mitochondrial levels of CoA. The results reported here and the proposed subcellular localization of some of the enzymes of the fruit fly CoA biosynthetic pathway, suggest that dPCoA may be synthesized and phosphorylated to CoA in the matrix, but it can also be transported by dPCoAC to the cytosol, where it may be phosphorylated to CoA by the monofunctional dPCoA kinase. Thus, dPCoAC may connect the cytosolic and mitochondrial reactions of the CoA biosynthetic pathway without allowing the two CoA pools to get in contact.

Links

PubMed Online version:10.1016/j.bbabio.2016.11.006

Keywords

Amino Acid Sequence; Animals; Biological Transport/physiology; Carrier Proteins/metabolism; Coenzyme A/metabolism; Cytosol/metabolism; Drosophila melanogaster/metabolism; Escherichia coli/metabolism; Kinetics; Mitochondria/metabolism; Mitochondrial Membrane Transport Proteins/metabolism; Protein Biosynthesis/physiology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Sequence Alignment

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:Q9VDL7

enables

GO:0015297: antiporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(CHEBI:16761)
  • has_input:(CHEBI:15468)

Seeded From UniProt

complete

DROME:Q9VDL7

located_in

GO:0032592: integral component of mitochondrial membrane

ECO:0000305: curator inference used in manual assertion

GO:0015297,GO:1990542

C

Seeded From UniProt

complete

DROME:Q9VDL7

involved_in

GO:1990542: mitochondrial transmembrane transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:Q9VDL7

enables

GO:0015297: antiporter activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_direct_input:(CHEBI:16761)
  • has_direct_input:(CHEBI:15468)

Seeded From UniProt

complete

DROME:Q9VDL7

part_of

GO:0032592: integral component of mitochondrial membrane

ECO:0000305: curator inference used in manual assertion

GO:0015297,GO:1990542

C

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.