GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:27746179

From GONUTS
Jump to: navigation, search
Citation

Urafuji, K and Arioka, M' (2016) Yor022c protein is a phospholipase A1 that localizes to the mitochondrial matrix. Biochem. Biophys. Res. Commun. '

Abstract

In mammals, three types of intracellular phospholipase A1 (iPLA1) enzymes have been characterized and are thought to be involved in various cellular processes such as phospholipid metabolism, organelle biogenesis, and membrane trafficking. In this study we analyzed the unique iPLA1-like protein, Yor022c, in the budding yeast Saccharomyces cerevisiae. By the mass spectrometry analysis, we demonstrate that Yor022c is actually a phospholipase displaying sn-1-specific activity toward phosphatidylcholine, phosphatidylethanolamine, and phosphatidic acid, generating 2-acyl lysophospholipids. GFP-fused Yor022c co-stained with the mitochondrial dye MitoTracker, indicating that, unlike its mammalian counterparts, it is a mitochondrial protein. Further biochemical fractionation experiment combined with protease sensitivity assay showed that Yor022c localizes to the mitochondrial matrix. Thus Yor022c is the first PLA1 putatively involved in the maintenance of sn-1 acyl chains of phospholipids in the mitochondrial inner membrane.

Links

PubMed Online version:10.1016/j.bbrc.2016.10.028

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

YEAST:YOR22

located_in

GO:0005759: mitochondrial matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:YOR22

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

  • has_input:(CHEBI:64482)|has_input:(CHEBI:16038)|has_input:(CHEBI:16337)

Seeded From UniProt

complete

YEAST:YOR22

GO:0008970: phosphatidylcholine 1-acylhydrolase activity

ECO:0000314:

F

The author used mass spectrometry analysis on Yor022c and three different synthetic phospholipids and found that Yor022c cleaved at specifically the sn-1-position indicating that it is a phosphatidylcholine 1-acylhydrolase (Figure 2).

complete
CACAO 12159

YEAST:YOR22

GO:0005759: mitochondrial matrix

ECO:0000314:

C

The author purified mitochondria and created three groups of mitochondria that were left intact, had their outer membranes ruptured, and had their entire membrane dissolved. The author then treated the groups of mitochondria with proteinase K and performed a western blot. It was found from the western blot that the only time Yor022c was degraded by proteinase K was when the entire mitochondrial membrane was dissolved indicating that Yor022c was localized to the mitochondrial matrix (Figure 4b).

complete
CACAO 12160

YEAST:YOR22

part_of

GO:0005759: mitochondrial matrix

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

YEAST:YOR22

enables

GO:0004620: phospholipase activity

ECO:0000314: direct assay evidence used in manual assertion

F

has_direct_input:(CHEBI:64482)|has_direct_input:(CHEBI:16038)|has_direct_input:(CHEBI:16337)

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.