PMID:26887841

Citation	Korol, N, Van den Bossche, A, Romaniuk, L, Noben, JP, Lavigne, R and Tovkach, F (2016) Experimental evidence for proteins constituting virion components and particle morphogenesis of bacteriophage ZF40. FEMS Microbiol. Lett. 363
Abstract	Bacteriophage ZF40 is the only currently available, temperate Myoviridae phage infecting the potato pathogen Pectobacterium carotovorum subsp. carotovorum. Despite its unusual tail morphology, its major tail sheath and tube proteins remained uncharacterized after the initial genome annotation. Using ESI tandem mass-spectrometry, 24 structural proteins of the ZF40 virion were identified, with a sequence coverage ranging between 15.8% and 87.8%. The putative function of 16 proteins could be elucidated based on secondary structure analysis and conservative domain searches. The experimental annotation of 35% of the encoded gene products within the structural region of the genome represents a complete view of the virion structure, which can serve as the basis for future structural analysis as a model phage.
Links	PubMed Online version:10.1093/femsle/fnw042
Keywords
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
9CAUD:H9C192		GO:0046798: viral portal complex	ECO:0007184:		C		Table 1 shows that protein gp42 is a capsid component in Pectobacterium phage ZF40 with a molecular weight of 51.92 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-PAGE. Uniprot: ZF40_0042, Putative portal protein	complete CACAO 13594
9CAUD:H9C193		GO:0098018: viral capsid, minor subunit	ECO:0007184:		C		Table 1 shows that protein gp43 is a component of Pectobacterium phage ZF40 with a molecular weight of 36.01 kDa as determined through ESI-tandem mass spectrometry. Figure 1D lane 3 shows that the protein was isolated through SDS-Page. Uniprot: ZF40_0043, Putative head protein	complete CACAO 13597
9CAUD:H9C1B7		GO:0098015: virus tail	ECO:0007184:		C		Table 1 shows that Elecrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative tail-fiber protein ZF40_0067 (gp67) is a component of Pectobacteriumphage ZF40 with a molecular weight of 71.51 kDa. Figure 1C shows that Putative tail fiber protein ZF40_0067 was isolated by SDS-PAGE in lane 2.	complete CACAO 13602
9CAUD:H9C194		GO:0019028: viral capsid	ECO:0007184:		C		Table 1 shows that protein gp44 is a capsid component of the Pectobacterium phage ZF40 with a molecular weight of 44.12 kDa as determined through ESI-tandem mass spectrometry. In figure 1D lane 3, it is shown that the protein was isolated through SDS-PAGE. Uniprot: ZF40_0044, Putative head protein	complete CACAO 13603
9CAUD:H9C1B3		GO:0098015: virus tail, baseplate	ECO:0007184:		C		Table 1 shows that Elecrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative baseplate protein ZF40_0063 (gp63) is a component of Pectobacteriumphage ZF40 with a molecular weight of 42.89 kDa. Figure 1C shows that Putative baseplate protein ZF0_0063 was isolated using SDS-PAGE in lane 2.	complete CACAO 13606
9CAUD:H9C196		GO:0098017: viral capsid, major subunit	ECO:0007184:		C		Table 1 shows that protein gp46 is a major capsid protein in the Pectobacterium phage ZF40 with a molecular weight of 34.35 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 shows that this protein was isolated through SDS-PAGE. Uniprot Name(s): Putative major capsid protein, ZF40_0046	complete CACAO 13605
9CAUD:H9C198		GO:0019012: virion	ECO:0007184:		C		Table 1 shows that protein gp48 is a component of the Pectobacterium phage ZF40 with a molecular weight of 18.74 kDa as determined through ESI-tandem mass spectrometry. In figure 1D, lane 3 displays the isolation of the protein using SDS-PAGE. Uniprot Name(s): Uncharacterized Protein, ZF40_0048	complete CACAO 13607
9CAUD:H9C1B2		GO:0098015: virus tail, baseplate	ECO:0007184:		C		Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Uncharacterized protein ZF40_0062 (gp62) is a component of Pectobacteriumphage ZF40 with a molecular weight of 15.02 kDa. Figure 1C shows that Uncharacterized protein ZF0_0062 was isolated using SDS-PAGE in lane 2.	complete CACAO 13611
9CAUD:H9C1B1		GO:0098015: virion	ECO:0007184:		C		Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Putative baseplate protein ZF40_0061 (gp61) is a component of Pectobacteriumphage ZF40 with a molecular weight of 24.51 kDa. Figure 1C shows that Putative baseplate protein ZF0_0061 was isolated using SDS-PAGE in lane 2.	complete CACAO 13613
9CAUD:H9C1B0		GO:0098015: virion	ECO:0007184:		C		Table 1 shows that Electrospray Ionization Mass Spectrometry (ESI-MS) was used to show that Uncharacterized protein ZF40_0060 (gp60) is a component of Pectobacteriumphage ZF40 with a molecular weight of 34.25 kDa. Figure 1C shows that Uncharacterized protein ZF0_0060 was isolated using SDS-PAGE in lane 2.	complete CACAO 13615
9CAUD:H9C199		GO:0098015: virus tail	ECO:0007184:		C		In Table 1 gp49/phage tail component (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is phage tail samples.	complete CACAO 13617
9CAUD:H9C1A2		GO:0098027: virus tail, sheath	ECO:0007184:		C		In Table 1 gp52/major tail sheath protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 3, of Figure 1D, which is identified as phage tail samples.	complete CACAO 13654
9CAUD:H9C1A3		GO:0098026: virus tail, tube	ECO:0007184:		C		In Table 1 gp53/putative tail tube protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 3, of Figure 1D, which is identified as phage tail samples.	complete CACAO 13655
9CAUD:H9C1A7		GO:0098015: virus tail	ECO:0007184:		C		In Table 1 gp57/tape measure protein/lysin (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is identified as phage tail samples.	complete
9CAUD:H9C1A8		GO:0098015: virus tail	ECO:0007184:		C		In Table 1 gp58/ tail protein (an uncharacterized protein) was shown to be part of the Bacteriophage ZF40 virus (also referenced Pectobacterium phage ZF40) through ESI-MS/MS. This protein was isolated in Lane 2, of Figure 1C, which is identified as phage tail samples.	complete

References

See Help:References for how to manage references in GONUTS.