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PMID:26804021

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Citation

Tashiro, S, Handa, T, Matsuda, A, Ban, T, Takigawa, T, Miyasato, K, Ishii, K, Kugou, K, Ohta, K, Hiraoka, Y, Masukata, H and Kanoh, J (2016) Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing. Nat Commun 7:10393

Abstract

A chromosome is composed of structurally and functionally distinct domains. However, the molecular mechanisms underlying the formation of chromatin structure and the function of subtelomeres, the telomere-adjacent regions, remain obscure. Here we report the roles of the conserved centromeric protein Shugoshin 2 (Sgo2) in defining chromatin structure and functions of the subtelomeres in the fission yeast Schizosaccharomyces pombe. We show that Sgo2 localizes at the subtelomeres preferentially during G2 phase and is essential for the formation of a highly condensed subtelomeric chromatin body 'knob'. Furthermore, the absence of Sgo2 leads to the derepression of the subtelomeric genes and premature DNA replication at the subtelomeric late origins. Thus, the subtelomeric specialized chromatin domain organized by Sgo2 represses both transcription and replication to ensure proper gene expression and replication timing.

Links

PubMed Online version:10.1038/ncomms10393

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:SGO2

is_active_in

GO:0000775: chromosome, centromeric region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:SGO2

is_active_in

GO:0099115: chromosome, subtelomeric region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:SGO2

part_of

GO:0000775: chromosome, centromeric region

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:SGO2

part_of

GO:0031933: telomeric heterochromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:SGO2

part_of

GO:1990421: subtelomeric heterochromatin

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0000085)

Seeded From UniProt

complete

SCHPO:SGO2

GO:1990421: subtelomeric heterochromatin

ECO:0000314:

C

ChIP–chip analysis of the genome-wide distribution of Sgo2-Flag in logarithmically growing wt cells. It is noteworthy that the probes used in the tiling array did not encompass regions outside the rDNA repeats on chromosome 3. (c) Magnification of the ChIP–chip data for the subtelomeric regions shown in b.

complete
CACAO 11221

SCHPO:SGO2

GO:1903467: negative regulation of mitotic DNA replication initiation

ECO:0000315:

P

Figure 7

complete
CACAO 11222

Notes

See also

References

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