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PMID:26723254

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Citation

Chung, TW, Park, MJ, Kim, HS, Choi, HJ and Ha, KT (2016) Integrin αVβ3 and αVβ5 are required for leukemia inhibitory factor-mediated the adhesion of trophoblast cells to the endometrial cells. Biochem. Biophys. Res. Commun. 469:936-40

Abstract

The embryo implantation including adhesion between trophoblast and endometrium is a crucial process for the successful pregnancy. LIF and adhesion molecules including integrins are known as significant factors for embryo implantation. However, the function of LIF on the regulation of adhesion molecule expression and promotion of trophoblast adhesion to endometrial cells has not been fully elucidated. Here we show that LIF significantly induced mRNA expression of ITGAV, ITGB3, and ITGB5 in endometrial cells, as evidenced by RT-PCR and qRT-PCR analysis. Based on the results from treatment of antagonist for LIF receptor (hLA), LIF positively regulates expression of integrin αV, β3, and β5, and adhesion of the human trophectoderm-derived JAr cells to endometrial Ishikawa cells. Furthermore, the adhesion between trophoblastic cells and LIF-stimulated endometrial cells was significantly reduced by neutralization of LIF-mediated integrin β3 and β5 expression on endometrial cell surface with integrin subunit β3 and β5 antibodies. Taken together, we firstly demonstrate that LIF enhances the adhesion of trophoblastic cells to endometrial cells by up-regulating expression of integrin heterodimer αVβ3 and αVβ5, indicating the promotion of endometrial receptivity for embryo implantation.

Links

PubMed Online version:10.1016/j.bbrc.2015.12.103

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:LIF

GO:0045944: positive regulation of transcription from RNA polymerase II promoter

ECO:0000314:

P

Figure 1 shows increased integrin expression (higher levels of integrin mRNA)

complete
CACAO 11522

HUMAN:LIF

GO:0033630: positive regulation of cell adhesion mediated by integrin

ECO:0000315:

P

As shown in Fig. 2B, LIF increased the adhesion of the human trophectoderm-derived JAr cells to endometrial Ishikawa cells. On the contrary, hLA diminished the adhesion of JAr cells to LIF-stimulated Ishikawa cells. These results suggest that the enhancement of integrin αV, β3, and β5 by LIF might be associated with the adhesion of trophblast to endometrium for embryo implantation. Figure 2A shows adhesion is dependent on integrins. (hLa inhibits LIF, hence IMP) Figure 3 uses antibodies to neutralize the integrins.

complete
CACAO 11523

HUMAN:LIF

GO:0045944: positive regulation of transcription from RNA polymerase II promoter

ECO:0000315:

P

Figure 2A Treatment of hLA (inhibitor of LIF) clearly resulted in the reduction of ITGAV, ITGB3 and ITGB5 expression levels induced by LIF in the Ishikawa cells

complete
CACAO 11524

Notes

See also

References

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