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PMID:26628744

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Citation

Jozwiak, A, Gutkowska, M, Gawarecka, K, Surmacz, L, Buczkowska, A, Lichocka, M, Nowakowska, J and Swiezewska, E (2015) POLYPRENOL REDUCTASE2 Deficiency Is Lethal in Arabidopsis Due to Male Sterility. Plant Cell 27:3336-53

Abstract

Dolichol is a required cofactor for protein glycosylation, the most common posttranslational modification modulating the stability and biological activity of proteins in all eukaryotic cells. We have identified and characterized two genes, PPRD1 and -2, which are orthologous to human SRD5A3 (steroid 5α reductase type 3) and encode polyprenol reductases responsible for conversion of polyprenol to dolichol in Arabidopsis thaliana. PPRD1 and -2 play dedicated roles in plant metabolism. PPRD2 is essential for plant viability; its deficiency results in aberrant development of the male gametophyte and sporophyte. Impaired protein glycosylation seems to be the major factor underlying these defects although disturbances in other cellular dolichol-dependent processes could also contribute. Shortage of dolichol in PPRD2-deficient cells is partially rescued by PPRD1 overexpression or by supplementation with dolichol. The latter has been discussed as a method to compensate for deficiency in protein glycosylation. Supplementation of the human diet with dolichol-enriched plant tissues could allow new therapeutic interventions in glycosylation disorders. This identification of PPRD1 and -2 elucidates the factors mediating the key step of the dolichol cycle in plant cells which makes manipulation of dolichol content in plant tissues feasible.

Links

PubMed PMC4707453 Online version:10.1105/tpc.15.00463

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:POED1

acts_upstream_of_or_within

GO:0016093: polyprenol metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:POED2

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:POED2

acts_upstream_of_or_within

GO:0006487: protein N-linked glycosylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:POED1

GO:0016229: steroid dehydrogenase activity

ECO:0000314:

F

Figures 1E- PPRD1 and PPRD2 Catalyze Reduction of Polyprenol to Dolichol in Vitro, which is an essential step in N-linked protein glycosylation. Polyprenol is a sterol derivative.

complete
CACAO 12117

ARATH:POED1

enables

GO:0003865: 3-oxo-5-alpha-steroid 4-dehydrogenase activity

ECO:0000316: genetic interaction evidence used in manual assertion

SGD:S000001311

F

Seeded From UniProt

complete

ARATH:POED1

involved_in

GO:0016093: polyprenol metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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