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PMID:26565907

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Citation

Esteves da Silva, M, Adrian, M, Schätzle, P, Lipka, J, Watanabe, T, Cho, S, Futai, K, Wierenga, CJ, Kapitein, LC and Hoogenraad, CC (2015) Positioning of AMPA Receptor-Containing Endosomes Regulates Synapse Architecture. Cell Rep 13:933-43

Abstract

Lateral diffusion in the membrane and endosomal trafficking both contribute to the addition and removal of AMPA receptors (AMPARs) at postsynaptic sites. However, the spatial coordination between these mechanisms has remained unclear, because little is known about the dynamics of AMPAR-containing endosomes. In addition, how the positioning of AMPAR-containing endosomes affects synapse organization and functioning has never been directly explored. Here, we used live-cell imaging in hippocampal neuron cultures to show that intracellular AMPARs are transported in Rab11-positive recycling endosomes, which frequently enter dendritic spines and depend on the microtubule and actin cytoskeleton. By using chemically induced dimerization systems to recruit kinesin (KIF1C) or myosin (MyosinV/VI) motors to Rab11-positive recycling endosomes, we controlled their trafficking and found that induced removal of recycling endosomes from spines decreases surface AMPAR expression and PSD-95 clusters at synapses. Our data suggest a mechanistic link between endosome positioning and postsynaptic structure and composition.

Links

PubMed Online version:10.1016/j.celrep.2015.09.062

Keywords

Actin Cytoskeleton/metabolism; Animals; Cells, Cultured; Dendritic Spines/metabolism; Dendritic Spines/ultrastructure; Endosomes/metabolism; Female; Kinesin/metabolism; Male; Mice; Mice, Inbred C57BL; Myosins/metabolism; Rats; Receptors, AMPA/genetics; Receptors, AMPA/metabolism; Synapses/metabolism; Synapses/ultrastructure; rab GTP-Binding Proteins/genetics; rab GTP-Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:RB11A

part_of

GO:0098837: postsynaptic recycling endosome

ECO:0005589: confocal microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RB11A

is_active_in

GO:0098837: postsynaptic recycling endosome

ECO:0005589: confocal microscopy evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098837: postsynaptic recycling endosome

ECO:0006063: over expression analysis evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RB11A

is_active_in

GO:0098837: postsynaptic recycling endosome

ECO:0006063: over expression analysis evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098978: glutamatergic synapse

ECO:0005589: confocal microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

is_active_in

GO:0098978: glutamatergic synapse

ECO:0005589: confocal microscopy evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098978: glutamatergic synapse

ECO:0006063: over expression analysis evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

is_active_in

GO:0098978: glutamatergic synapse

ECO:0006063: over expression analysis evidence used in manual assertion

C

  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

RAT:RB11A

located_in

GO:0098837: postsynaptic recycling endosome

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098978: glutamatergic synapse

ECO:0006063: over expression analysis evidence used in manual assertion

C

part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098978: glutamatergic synapse

ECO:0005589: confocal microscopy evidence used in manual assertion

C

part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098837: postsynaptic recycling endosome

ECO:0006063: over expression analysis evidence used in manual assertion

C

  • part_of:(GO:0098978)
  • part_of:(UBERON:0000061)
  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

HUMAN:RB11A

part_of

GO:0098837: postsynaptic recycling endosome

ECO:0005589: confocal microscopy evidence used in manual assertion

C

  • part_of:(GO:0098978)
  • part_of:(UBERON:0000061)
  • part_of:(UBERON:0002421)

Seeded From UniProt

complete

Notes

See also

References

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