PMID:2644218

Citation	Jacobson, MR, Brigle, KE, Bennett, LT, Setterquist, RA, Wilson, MS, Cash, VL, Beynon, J, Newton, WE and Dean, DR (1989) Physical and genetic map of the major nif gene cluster from Azotobacter vinelandii. J. Bacteriol. 171:1017-27
Abstract	Determination of a 28,793-base-pair DNA sequence of a region from the Azotobacter vinelandii genome that includes and flanks the nitrogenase structural gene region was completed. This information was used to revise the previously proposed organization of the major nif cluster. The major nif cluster from A. vinelandii encodes 15 nif-specific genes whose products bear significant structural identity to the corresponding nif-specific gene products from Klebsiella pneumoniae. These genes include nifH, nifD, nifK, nifT, nifY, nifE, nifN, nifX, nifU, nifS, nifV, nifW, nifZ, nifM, and nifF. Although there are significant spatial differences, the identified A. vinelandii nif-specific genes have the same sequential arrangement as the corresponding nif-specific genes from K. pneumoniae. Twelve other potential genes whose expression could be subject to nif-specific regulation were also found interspersed among the identified nif-specific genes. These potential genes do not encode products that are structurally related to the identified nif-specific gene products. Eleven potential nif-specific promoters were identified within the major nif cluster, and nine of these are preceded by an appropriate upstream activator sequence. A + T-rich regions were identified between 8 of the 11 proposed nif promoter sequences and their upstream activator sequences. Site-directed deletion-and-insertion mutagenesis was used to establish a genetic map of the major nif cluster.
Links	PubMed PMC209696
Keywords	Amino Acid Sequence; Azotobacter/genetics; Base Sequence; Cloning, Molecular; Codon/genetics; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Multigene Family; Nitrogen Fixation/genetics; Plasmids; Restriction Mapping; Species Specificity
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
AZOVI:NIFK		GO:0009399: nitrogen fixation	ECO:0000315:		P		Table 4. Nitrogen fixation function of Nif K gene was determined by comparison with mutant strains for gene expression.	complete CACAO 2819
AZOVI:NIFK	involved_in	GO:0009399: nitrogen fixation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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