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PMID:2644194

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Citation

Mat-Jan, F, Alam, KY and Clark, DP (1989) Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase. J. Bacteriol. 171:342-8

Abstract

Mutants of Escherichia coli deficient in the fermentative NAD-linked lactate dehydrogenase (ldh) have been isolated. These mutants showed no growth defects under anaerobic conditions unless present together with a defect in pyruvate formate lyase (pfl). Double mutants (pfl ldh) were unable to grow anaerobically on glucose or other sugars even when supplemented with acetate, whereas pfl mutants can do so. The ldh mutation was found to map at 30.5 min on the E. coli chromosome. The ldh mutant FMJ39 showed no detectable lactate dehydrogenase activity and produced no lactic acid from glucose under anaerobic conditions as estimated by in vivo nuclear magnetic resonance measurements. We also found that in wild-type strains the fermentative lactate dehydrogenase was conjointly induced by anaerobic conditions and an acidic pH. Despite previous findings that phosphate concentrations affect the proportion of lactic acid produced during fermentation, we were unable to find any intrinsic effect of phosphate on lactate dehydrogenase activity, apart from the buffering effect of this ion.

Links

PubMed PMC209593

Keywords

Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; Escherichia coli/enzymology; Escherichia coli/genetics; Fermentation; Genes; Genes, Bacterial; Genotype; L-Lactate Dehydrogenase/genetics; L-Lactate Dehydrogenase/metabolism; Mutation; Restriction Mapping

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:LDHD

acts_upstream_of_or_within

GO:0019664: mixed acid fermentation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ECOLI:LDHD

GO:0008720: D-lactate dehydrogenase activity

ECO:0000316:

UniProtKB:P09373


F

Table 3. LDH Mutants of E. Coli and their LDH activity Figure 1. Wild type and mutant NMR of fermentation products

complete

ECOLI:LDHD

GO:0019661: glucose catabolic process to lactate via pyruvate

ECO:0000316:

UniProtKB:P09373


P

Figure 1. NMR analysis of fermentation products

complete

ECOLI:LDHD

enables

GO:0008720: D-lactate dehydrogenase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ECOLI:LDHD

involved_in

GO:0019664: mixed acid fermentation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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