PMID:26310304

Citation	Jeon, S, Lim, S, Ha, J and Kim, J (2015) Identification of Psk2, Skp1, and Tub4 as trans-acting factors for uORF-containing ROK1 mRNA in Saccharomyces cerevisiae. J. Microbiol. 53:616-22
Abstract	Rok1, a DEAD-box RNA helicase, is involved in rRNA processing and the control of cell cycle progression in Saccharomyces cerevisiae. Rok1 protein expression is cell cycle-regulated, declining at G1/S and increasing at G2. The downregulation of Rok1 expression in G1/S phase is mediated by the inhibitory action of two upstream open reading frames (uORFs) in the ROK1 5'-untranslated region (5'UTR). We identified Psk2 (PAS kinase), Skp1 (kinetochore protein) and Tub4 (γ-tubulin protein) as ROK1 5'UTR-interacting proteins using yeast three-hybrid system. A deletion analysis of PSK2 or inactivation of temperature-sensitive alleles of SKP1 and TUB4 revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1. This repression appeared to be mediated through the ROK1 uORF1. In contrast, Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation. These results suggest that 5'UTR-interacting proteins, identified using three hybrid screening, are important for uORF-mediated regulation of Rok1 protein expression.
Links	PubMed Online version:10.1007/s12275-015-5389-5
Keywords	5' Untranslated Regions; Cell Cycle; DEAD-box RNA Helicases/genetics; Down-Regulation; F-Box Proteins/genetics; Intracellular Signaling Peptides and Proteins/genetics; Open Reading Frames; Protein Biosynthesis; Protein-Serine-Threonine Kinases/genetics; SKP Cullin F-Box Protein Ligases/genetics; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/growth & development; Saccharomyces cerevisiae Proteins/genetics; Trans-Activators/genetics; Tubulin/genetics; Two-Hybrid System Techniques
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:SKP1	involved_in	GO:2000766: negative regulation of cytoplasmic translation	ECO:0000315: mutant phenotype evidence used in manual assertion		P	has_input:(UniProtKB:P45818)	Seeded From UniProt	complete
YEAST:TBG	involved_in	GO:2000767: positive regulation of cytoplasmic translation	ECO:0000315: mutant phenotype evidence used in manual assertion		P	has_input:(UniProtKB:P45818)	Seeded From UniProt	complete
YEAST:PSK2	involved_in	GO:2000766: negative regulation of cytoplasmic translation	ECO:0000315: mutant phenotype evidence used in manual assertion		P	has_input:(UniProtKB:P45818)	Seeded From UniProt	complete
YEAST:SKP1	involved_in	GO:2000766: negative regulation of cytoplasmic translation	ECO:0000315: mutant phenotype evidence used in manual assertion		P	has_direct_input:(UniProtKB:P45818)	Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.