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PMID:26161528

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Citation

Girard, C, Chelysheva, L, Choinard, S, Froger, N, Macaisne, N, Lemhemdi, A, Lehmemdi, A, Mazel, J, Crismani, W and Mercier, R (2015) AAA-ATPase FIDGETIN-LIKE 1 and Helicase FANCM Antagonize Meiotic Crossovers by Distinct Mechanisms. PLoS Genet. 11:e1005369

Abstract

Meiotic crossovers (COs) generate genetic diversity and are critical for the correct completion of meiosis in most species. Their occurrence is tightly constrained but the mechanisms underlying this limitation remain poorly understood. Here we identified the conserved AAA-ATPase FIDGETIN-LIKE-1 (FIGL1) as a negative regulator of meiotic CO formation. We show that Arabidopsis FIGL1 limits CO formation genome-wide, that FIGL1 controls dynamics of the two conserved recombinases DMC1 and RAD51 and that FIGL1 hinders the interaction between homologous chromosomes, suggesting that FIGL1 counteracts DMC1/RAD51-mediated inter-homologue strand invasion to limit CO formation. Further, depleting both FIGL1 and the previously identified anti-CO helicase FANCM synergistically increases crossover frequency. Additionally, we showed that the effect of mutating FANCM on recombination is much lower in F1 hybrids contrasting from the phenotype of inbred lines, while figl1 mutation equally increases crossovers in both contexts. This shows that the modes of action of FIGL1 and FANCM are differently affected by genomic contexts. We propose that FIGL1 and FANCM represent two successive barriers to CO formation, one limiting strand invasion, the other disassembling D-loops to promote SDSA, which when both lifted, leads to a large increase of crossovers, without impairing meiotic progression.

Links

PubMed PMC4498898 Online version:10.1371/journal.pgen.1005369

Keywords

ATPases Associated with Diverse Cellular Activities; Adenosine Triphosphatases/genetics; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Cell Cycle Proteins/genetics; Crossing Over, Genetic/genetics; DNA Helicases/genetics; DNA Repair/genetics; Genetic Variation/genetics; Meiosis/genetics; Microtubule-Associated Proteins; Rad51 Recombinase/genetics; Rec A Recombinases/genetics; Recombination, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:F4HYE4

acts_upstream_of_or_within

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT3G27120

P

Seeded From UniProt

complete

ARATH:FIGL1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:FIGL1

acts_upstream_of_or_within

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:FIGL1

acts_upstream_of_or_within

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G35530

P

Seeded From UniProt

complete

ARATH:FIGL1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:FIGL1

involved_in

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:FIGL1

involved_in

GO:0045128: negative regulation of reciprocal meiotic recombination

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G35530

P

Seeded From UniProt

complete

Notes

See also

References

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