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PMID:26147377

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Citation

Carlotto, N, Wirth, S, Furman, N, Ferreyra Solari, N, Ariel, F, Crespi, M and Kobayashi, K (2016) The chloroplastic DEVH-box RNA helicase INCREASED SIZE EXCLUSION LIMIT 2 involved in plasmodesmata regulation is required for group II intron splicing. Plant Cell Environ. 39:165-73

Abstract

INCREASED SIZE EXCLUSION LIMIT 2 (ISE2) encodes a putative DEVH-box RNA helicase originally identified through a genetic screening for Arabidopsis mutants altered in plasmodesmata (PD) aperture. Depletion of ISE2 also affects chloroplasts activity, decreases accumulation of photosynthetic pigments and alters expression of photosynthetic genes. In this work, we show the chloroplast localization of ISE2 and decipher its role in plastidic RNA processing and, consequently, PD function. Group II intron-containing RNAs from chloroplasts exhibit defective splicing in ise2 mutants and ISE2-silenced plants, compromising plastid viability. Furthermore, RNA immunoprecipitation suggests that ISE2 binds in vivo to several splicing-regulated RNAs. Finally, we show that the chloroplast clpr2 mutant (defective in a subunit of a plastidic Clp protease) also exhibits abnormal PD function during embryogenesis, supporting the idea that chloroplast RNA processing is required to regulate cell-cell communication in plants.

Links

PubMed Online version:10.1111/pce.12603

Keywords

Arabidopsis/enzymology; Arabidopsis/genetics; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Biological Transport; Chloroplasts/enzymology; DEAD-box RNA Helicases/genetics; DEAD-box RNA Helicases/metabolism; Gene Expression Regulation, Plant; Genes, Reporter; Introns/genetics; Mutation; Photosynthesis; Plants, Genetically Modified; Plasmodesmata/metabolism; RNA Helicases/genetics; RNA Helicases/metabolism; RNA Splicing; RNA, Chloroplast/genetics; RNA, Chloroplast/metabolism; Seedlings/drug effects; Seedlings/enzymology; Seedlings/genetics; Seedlings/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:ISE2

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ISE2

part_of

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:ISE2

involved_in

GO:0000373: Group II intron splicing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:ISE2

enables

GO:0003723: RNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:ISE2

involved_in

GO:0006397: mRNA processing

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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