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PMID:26023161

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Citation

Brosseau, C and Moffett, P (2015) Functional and Genetic Analysis Identify a Role for Arabidopsis ARGONAUTE5 in Antiviral RNA Silencing. Plant Cell 27:1742-54

Abstract

RNA silencing functions as an antiviral defense through the action of DICER-like (DCL) and ARGONAUTE (AGO) proteins. In turn, plant viruses have evolved strategies to counteract this defense mechanism, including the expression of suppressors of RNA silencing. Potato virus X (PVX) does not systemically infect Arabidopsis thaliana Columbia-0, but is able to do so effectively in mutants lacking at least two of the four Arabidopsis DCL proteins. PVX can also infect Arabidopsis ago2 mutants, albeit less effectively than double DCL mutants, suggesting that additional AGO proteins may mediate anti-viral defenses. Here we show, using functional assays, that all Arabidopsis AGO proteins have the potential to target PVX lacking its viral suppressor of RNA silencing (VSR), P25, but that only AGO2 and AGO5 are able to target wild-type PVX. However, P25 directly affects only a small subset of AGO proteins, and we present evidence indicating that its protective effect is mediated by precluding AGO proteins from accessing viral RNA, as well as by directly inhibiting the RNA silencing machinery. In agreement with functional assays, we show that Potexvirus infection induces AGO5 expression and that both AGO2 and AGO5 are required for full restriction of PVX infection in systemic tissues of Arabidopsis.

Links

PubMed PMC4498209 Online version:10.1105/tpc.15.00264

Keywords

Arabidopsis/genetics; Arabidopsis/physiology; Arabidopsis/virology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/physiology; Argonaute Proteins/genetics; Argonaute Proteins/physiology; Cell Cycle Proteins/genetics; Cell Cycle Proteins/physiology; Plant Viruses/genetics; Plant Viruses/physiology; Potexvirus/genetics; Potexvirus/physiology; RNA Interference/physiology; RNA, Small Interfering/genetics; RNA, Small Interfering/physiology; RNA, Viral/genetics; RNA-Binding Proteins/genetics; RNA-Binding Proteins/physiology; Ribonuclease III/genetics; Ribonuclease III/physiology; Tobacco/virology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:AGO6

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO9

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL3

involved_in

GO:0051607: defense response to virus

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT3G03300
AGI_LocusCode:At5G20320

P

Seeded From UniProt

complete

ARATH:AGO3

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO5

part_of

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:AGO5

involved_in

GO:0009615: response to virus

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO5

involved_in

GO:0009814: defense response, incompatible interaction

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO5

involved_in

GO:0060145: viral gene silencing in virus induced gene silencing

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT1G31280

P

Seeded From UniProt

complete

ARATH:AGO4

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO10

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:DCL4

involved_in

GO:0051607: defense response to virus

ECO:0000316: genetic interaction evidence used in manual assertion

AGI_LocusCode:AT3G03300
AGI_LocusCode:AT3G43920

P

Seeded From UniProt

complete

ARATH:AGO7

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AGO1

involved_in

GO:0051607: defense response to virus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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