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PMID:25913773

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Citation

Zschiesche, W, Barth, O, Daniel, K, Böhme, S, Rausche, J and Humbeck, K (2015) The zinc-binding nuclear protein HIPP3 acts as an upstream regulator of the salicylate-dependent plant immunity pathway and of flowering time in Arabidopsis thaliana. New Phytol. 207:1084-96

Abstract

Biotic and abiotic stress responses of plants are linked to developmental programs. Proteins involved in different signaling pathways are the molecular basis of this concerted interplay. In our study, we show that Arabidopsis thaliana HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN3 (HIPP3; At5g60800) acts as an upstream regulator of stress- and development-related regulatory networks. Localization, metal-binding and stress-responsive gene expression of HIPP3 were analyzed via microscopy, protein and inductively coupled plasma (ICP)-MS analyses and quantitative real-time PCR. In addition, transcriptome and phenotype analyses of plants overexpressing HIPP3 were used to unravel its function. Our data show that HIPP3 is a nuclear, zinc-binding protein. It is repressed during drought stress and abscisic acid (ABA) treatment and, similar to other pathogen-related genes, is induced after infection with Pseudomonas syringae pv. tomato. HIPP3 overexpression affects the regulation of > 400 genes. Strikingly, most of these genes are involved in pathogen response, especially in the salicylate pathway. In addition, many genes of abiotic stress responses and seed and flower development are affected by HIPP3 overexpression. Plants overexpressing HIPP3 show delayed flowering. We conclude that HIPP3 acts via its bound zinc as an upstream regulator of the salicylate-dependent pathway of pathogen response and is also involved in abiotic stress responses and seed and flower development.

Links

PubMed Online version:10.1111/nph.13419

Keywords

Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/immunology; Arabidopsis/microbiology; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Carrier Proteins/genetics; Carrier Proteins/metabolism; Flowers/drug effects; Flowers/physiology; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes, Plant; Models, Biological; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Plant Immunity/drug effects; Protein Transport/drug effects; Pseudomonas syringae/physiology; RNA, Messenger/genetics; RNA, Messenger/metabolism; Salicylates/pharmacology; Spectrophotometry, Atomic; Subcellular Fractions/metabolism; Time Factors

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:HIP3

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:HIP3

located_in

GO:0005730: nucleolus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:HIP3

involved_in

GO:0009910: negative regulation of flower development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:HIP3

enables

GO:0008270: zinc ion binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:HIP3

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:HIP3

part_of

GO:0005730: nucleolus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:HIP3

GO:0005730: nucleus

ECO:0000314:

C

Organism: Arabidopsis Thaliana Protein: HIPP3//HIPP3 Figure 1: "After transformation with the smRS-GFP-HIPP3 construct, a clear green fluorescence is detected in the nucleus and especially in the nucleoli, indicating the nuclear localization of HIPP3."

complete
CACAO 12849

Notes

See also

References

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