GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:25675407

From GONUTS
Jump to: navigation, search
Citation

Tang, MC, Jacobs, SA, Mattiske, DM, Soh, YM, Graham, AN, Tran, A, Lim, SL, Hudson, DF, Kalitsis, P, O'Bryan, MK, Wong, LH and Mann, JR (2015) Contribution of the two genes encoding histone variant h3.3 to viability and fertility in mice. PLoS Genet. 11:e1004964

Abstract

Histones package DNA and regulate epigenetic states. For the latter, probably the most important histone is H3. Mammals have three near-identical H3 isoforms: canonical H3.1 and H3.2, and the replication-independent variant H3.3. This variant can accumulate in slowly dividing somatic cells, replacing canonical H3. Some replication-independent histones, through their ability to incorporate outside S-phase, are functionally important in the very slowly dividing mammalian germ line. Much remains to be learned of H3.3 functions in germ cell development. Histone H3.3 presents a unique genetic paradigm in that two conventional intron-containing genes encode the identical protein. Here, we present a comprehensive analysis of the developmental effects of null mutations in each of these genes. H3f3a mutants were viable to adulthood. Females were fertile, while males were subfertile with dysmorphic spermatozoa. H3f3b mutants were growth-deficient, dying at birth. H3f3b heterozygotes were also growth-deficient, with males being sterile because of arrest of round spermatids. This sterility was not accompanied by abnormalities in sex chromosome inactivation in meiosis I. Conditional ablation of H3f3b at the beginning of folliculogenesis resulted in zygote cleavage failure, establishing H3f3b as a maternal-effect gene, and revealing a requirement for H3.3 in the first mitosis. Simultaneous ablation of H3f3a and H3f3b in folliculogenesis resulted in early primary oocyte death, demonstrating a crucial role for H3.3 in oogenesis. These findings reveal a heavy reliance on H3.3 for growth, gametogenesis, and fertilization, identifying developmental processes that are particularly susceptible to H3.3 deficiency. They also reveal partial redundancy in function of H3f3a and H3f3b, with the latter gene being generally the most important.

Links

PubMed PMC4335506 Online version:10.1371/journal.pgen.1004964

Keywords

Animals; Cell Survival/genetics; Chromatin/genetics; DNA Replication/genetics; Female; Fertility/genetics; Fetus; Histones/genetics; Male; Meiosis/genetics; Mice; Oocytes/growth & development; Oogenesis; Spermatocytes/growth & development; Spermatocytes/pathology; Spermatozoa/growth & development; Spermatozoa/pathology; Zygote

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:H2AX

located_in

GO:0001741: XY body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SYCP3

located_in

GO:0000800: lateral element

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0007338: single fertilization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474378

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0007338: single fertilization

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474377

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0007286: spermatid development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474378

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0007283: spermatogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474378

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0007283: spermatogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474377

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0008584: male gonad development

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474378

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0048477: oogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1101768

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0048477: oogenesis

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:1097686

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0035264: multicellular organism growth

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474378

P

Seeded From UniProt

complete

MOUSE:H33

acts_upstream_of_or_within

GO:0035264: multicellular organism growth

ECO:0000315: mutant phenotype evidence used in manual assertion

MGI:MGI:5474377

P

Seeded From UniProt

complete

MOUSE:SYCP1

located_in

GO:0000795: synaptonemal complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:H1FNT

located_in

GO:0000785: chromatin

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:SYCP1

part_of

GO:0000795: synaptonemal complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:H2AX

part_of

GO:0001741: XY body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.