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PMID:2542268

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Citation

Liou, GI, Ma, DP, Yang, YW, Geng, L, Zhu, C and Baehr, W (1989) Human interstitial retinoid-binding protein. Gene structure and primary structure. J. Biol. Chem. 264:8200-6

Abstract

Interstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. We have used cDNA clones encoding human IRBP to isolate a 15-kilobase genomic fragment that encompasses the complete human IRBP gene. The IRBP gene spans more than 11 kilobases and is interrupted by three introns, all of which are positioned near the 3'-end of the coding sequence. The 3741-base pair coding region of IRBP appears to have been generated by quadruplication of an approximately 900 base pair long ancestral gene. The deduced amino acid sequence predicts a mature protein of 1,230 residues (calculated molecular weight 133,000). The protein sequence can be aligned into four homologous segments, each consisting of about 300 residues. Sequence similarity between segments is as high as 60% when conservative substitutions are taken into account. Two putative N-linked glycosylation sites are located in highly conserved domains in the center of the first and second segment of IRBP. A domain consisting of 41 residues at the COOH-terminal end of the third segment has 15 matching residues (38%) with an intradiscal loop of rhodopsin, a retinal-binding protein in rod photoreceptors.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Cattle; Codon; DNA Probes; DNA Restriction Enzymes; Endonucleases; Exons; Eye Proteins; Humans; Introns; Molecular Sequence Data; Nucleic Acid Hybridization; Protein Biosynthesis; RNA, Messenger/genetics; Retina/analysis; Retinol-Binding Proteins/genetics; Sequence Homology, Nucleic Acid; Single-Strand Specific DNA and RNA Endonucleases; Transcription, Genetic

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:RET3

enables

GO:0005501: retinoid binding

ECO:0000304: author statement supported by traceable reference used in manual assertion

F

Seeded From UniProt

complete

HUMAN:RET3

involved_in

GO:0006629: lipid metabolic process

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete

HUMAN:RET3

involved_in

GO:0007601: visual perception

ECO:0000304: author statement supported by traceable reference used in manual assertion

P

Seeded From UniProt

complete


See also

References

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