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PMID:2540956
Citation |
Lebovitz, RM, Takeyasu, K and Fambrough, DM (1989) Molecular characterization and expression of the (Na+ + K+)-ATPase alpha-subunit in Drosophila melanogaster. EMBO J. 8:193-202 |
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Abstract |
The (Na+ + K+)-ATPase (sodium pump) is an ouabain-sensitive, electrogenic ion pump responsible for maintaining the balance of sodium and potassium ions in almost all animal cells. Robust, ouabain-sensitive rubidium uptake, indicative of the sodium pump, was found in tissue-cultured Drosophila cells, and both larvae and adults die when fed a diet containing ouabain. A monoclonal antibody to the avian sodium pump alpha-subunit was found to cross-react with the Drosophila sodium pump alpha-subunit. Immunofluorescence microscopy was used to obtain a semi-quantitative view of the expression of the sodium pump in Drosophila tissues: high levels of the sodium pump were detected in malpighian tubules, indirect flight muscles and tubular muscles, and throughout the nervous system. The cDNA encoding this sodium pump alpha-subunit in Drosophila melanogaster was cloned, sequenced and expressed in mouse L cells. At the amino acid level, its deduced sequence of 1038 residues (the first such sequence for an invertebrate) is approximately 80% similar to alpha-subunit sequences reported for vertebrates. Only one gene was found in Drosophila, located on the third chromosome at position 93B. A restriction site polymorphism has been found, and several mutations exist that may involve the alpha-subunit gene. |
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Keywords |
Amino Acid Sequence; Animals; Base Sequence; Cells, Cultured; Chromosome Mapping; DNA/genetics; Drosophila melanogaster/enzymology; Drosophila melanogaster/genetics; L Cells (Cell Line); Mice; Molecular Sequence Data; Ouabain/pharmacology; RNA, Messenger/analysis; Recombinant Fusion Proteins/biosynthesis; Sodium-Potassium-Exchanging ATPase/antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase/biosynthesis; Sodium-Potassium-Exchanging ATPase/genetics |
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Significance
Annotations
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