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PMID:25397698

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Citation

Wang, C, Chen, Y, Deng, H, Gao, S and Li, L (2015) Rbm46 regulates trophectoderm differentiation by stabilizing Cdx2 mRNA in early mouse embryos. Stem Cells Dev. 24:904-15

Abstract

Blastocyst formation represents the first lineage specification by segregation of the trophectoderm from the inner cell mass in early embryonic development. Transcriptional regulation of Cdx2, which is selectively expressed in and essential for the specification of trophectoderm, has been extensively studied. However, post-transcriptional regulation of Cdx2 remains largely unknown. In this study, we report that RNA-binding protein motif 46 (Rbm46), an RNA-binding motif protein with unknown function, directly binds to and stabilizes Cdx2 mRNA in early mouse embryos. In addition, knockdown of Rbm46 using RNA interference downregulated the majority of trophectoderm markers in mouse embryonic stem cells and blocked the allocation of blastomere cells to the trophectoderm in mouse embryos. Our study revealed a novel mechanism by which Rbm46 regulates trophectoderm specification through stabilizing Cdx2 mRNA in early mouse embryos.

Links

PubMed Online version:10.1089/scd.2014.0323

Keywords

Animals; CDX2 Transcription Factor; Cells, Cultured; Ectoderm/cytology; Ectoderm/metabolism; Embryonic Stem Cells/cytology; Embryonic Stem Cells/metabolism; Homeodomain Proteins/genetics; Homeodomain Proteins/metabolism; Mice; Mice, Inbred C57BL; Protein Binding; RNA Stability; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA-Binding Proteins/genetics; RNA-Binding Proteins/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:PO5F1

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CDX2

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:CDX2

involved_in

GO:0001829: trophectodermal cell differentiation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:CDX2

involved_in

GO:0001829: trophectodermal cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RBM46

enables

GO:0003723: RNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

MOUSE:RBM46

involved_in

GO:0048255: mRNA stabilization

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:RBM46

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:RBM46

involved_in

GO:0001829: trophectodermal cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:NANOG

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:PO5F1

part_of

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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