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PMID:25049409

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Citation

Chung, IY, Jang, HJ, Bae, HW and Cho, YH (2014) A phage protein that inhibits the bacterial ATPase required for type IV pilus assembly. Proc. Natl. Acad. Sci. U.S.A. 111:11503-8

Abstract

Type IV pili (TFPs) are required for bacterial twitching motility and for phage infection in the opportunistic human pathogen Pseudomonas aeruginosa. Here we describe a phage-encoded protein, D3112 protein gp05 (hereafter referred to as Tip, representing twitching inhibitory protein), whose expression is necessary and sufficient to mediate the inhibition of twitching motility. Tip interacts with and blocks the activity of bacterial-encoded PilB, the TFP assembly/extension ATPase, at an internal 40-aa region unique to PilB. Tip expression results in the loss of surface piliation. Based on these observations and the fact that many P. aeruginosa phages require TFPs for infection, Tip-mediated twitching inhibition may represent a generalized strategy for superinfection exclusion. Moreover, because TFPs are required for full virulence, PilB may be an attractive target for the development of novel antiinfectives.

Links

PubMed PMC4128137 Online version:10.1073/pnas.1403537111

Keywords

Adenosine Triphosphatases/antagonists & inhibitors; Adenosine Triphosphatases/metabolism; Bacterial Proteins/metabolism; Bacteriophages/genetics; Bacteriophages/metabolism; Escherichia coli/metabolism; Fimbriae, Bacterial/metabolism; Genes, Viral; Humans; Protein Binding; Protein Interaction Domains and Motifs; Protein Interaction Mapping; Protein Transport; Pseudomonas aeruginosa/metabolism; Viral Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:FMPO

GO:0005829: cytosol

ECO:0000314:

C

Figure 4b

complete
CACAO 10228

PSEAE:FMPO

GO:0009986: cell surface

ECO:0000314:

C

Fig 4b

complete
CACAO 10260

PSEAE:FMPO

part_of

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:FMPO

part_of

GO:0009986: cell surface

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:PILB

involved_in

GO:0048870: cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PILB

involved_in

GO:0043107: type IV pilus-dependent motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:PILB

GO:0048870: cell motility

ECO:0000315:

P

Figure 3c

complete
CACAO 10229

PSEAE:PILB

GO:0043107: type IV pilus-dependent motility

ECO:0000315:

P

Figure 3b and c shows the effects of truncated pilb genes. They are unable to restore the twitching ability to the pilB mutant,which implies the deleted regions are necessary for biogenesis of the pili

complete
CACAO 10452

BPD31:Q6TM97

involved_in

GO:0039666: virion attachment to host cell pilus

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPD31:Q6TM97

involved_in

GO:1903652: modulation by virus of host cytoplasmic transport

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPD31:Q6TM97

GO:0044068: modulation by symbiont of host cellular process

ECO:0000315:

P

Fig. 1c NTR:11338 modulation by virus of host cell type IV motility

complete
CACAO 10453

BPD31:Q6TM97

GO:0039666: virion attachment to host cell pilus

ECO:0000314:

P

Figure 4A

complete
CACAO 10459

BPD31:Q6TM97

GO:1903652: modulation by virus of host cytoplasmic transport

ECO:0000314:

P

Fig.4c

complete
CACAO 10460

BPD31:Q6TM97

GO:0098669: superinfection exclusion

ECO:0007040: plaque assay evidence used in manual assertion

P

other:involved_in

Figure 1B

complete

Notes

See also

References

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