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Sugimoto, N, Mitoma, H, Kim, T, Hanabuchi, S and Liu, YJ (2014) Helicase proteins DHX29 and RIG-I cosense cytosolic nucleic acids in the human airway system. Proc. Natl. Acad. Sci. U.S.A. 111:7747-52


The recognition of cytoplasmic nucleic acid is critical for innate immune responses against microbial infection and is responsible for autoimmunity induced by dead cells. Here, we report the identification of a unique cytosolic nucleic acid cosensor in human airway epithelial cells and fibroblasts: DEAH (Asp-Glu-Ala-His) box polypeptide 29 (DHX29), a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family. Knocking down DHX29 by siRNA attenuated the ability of cells to mount type I IFN and IL-6 in response to cytosolic nucleic acids and various viruses by blocking the activation of interferon regulatory factor 3 and NF-κB-p65. The cytosolic nucleic acid sensing by DHX29 in human epithelial cells and fibroblasts is independent of stimulator of interferon genes but is dependent on retinoic acid-inducible gene 1 (RIG-I) and mitochondrial antiviral signaling protein (MAVS). DHX29 binds directly to nucleic acids and interacts with RIG-I and MAVS through its helicase 1 domain, activating the RIG-I-MAVS-dependent cytosolic nucleic acid response. These results suggest that DHX29 is a cytosolic nucleic acid cosensor that triggers RIG-I/MAVS-dependent signaling pathways. This study will have important implications in drug and vaccine design for control of viral infections and viral-induced pathology in the airway.


PubMed PMC4040624 Online version:10.1073/pnas.1400139111


Cytosol/metabolism; DEAD Box Protein 58; DEAD-box RNA Helicases/metabolism; DNA, Complementary; Humans; Immunity, Innate/immunology; Nucleic Acids/genetics; Nucleic Acids/metabolism; Plasmids/genetics; RNA Helicases/metabolism; RNA Interference; Real-Time Polymerase Chain Reaction; Respiratory Mucosa/metabolism; Sequence Analysis, DNA; Virus Diseases/immunology; Viruses/genetics



Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status


GO:0009615: detection of virus



Fig. 2I, and J show that knockdown DHX29 expression shows a reduced production of immune response peptides IFN-β and IL-6 upon viral stimulation. The protein is referred to as DHX29 in the paper and is specifically mentioned to be human DHX29.

CACAO 12280


See also


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