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PMID:24768539
| Citation |
Yuan, WC, Lee, YR, Lin, SY, Chang, LY, Tan, YP, Hung, CC, Kuo, JC, Liu, CH, Lin, MY, Xu, M, Chen, ZJ and Chen, RH (2014) K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. Mol. Cell 54:586-600 |
|---|---|
| Abstract |
Ubiquitin chains are formed as structurally distinct polymers via different linkages, and several chain types including K33-linkage remain uncharacterized. Here, we describe a role for K33-polyubiquitination in protein trafficking. We show that the Cullin 3 (Cul3) substrate adaptor KLHL20 is localized to the trans-Golgi network (TGN) and is important for post-Golgi trafficking by promoting the biogenesis of TGN-derived transport carriers. The Cul3-KLHL20 ubiquitin E3 ligase catalyzes a nondegradable, K33-linked polyubiquitination on coronin 7 (Crn7), which facilitates Crn7 targeting to TGN through a ubiquitin-dependent interaction with Eps15. Blockage of K33-chain formation, Crn7 ubiquitination, or disruption of Crn7-Eps15 interaction impairs TGN-pool F-actin assembly, a process essential for generating transport carriers. Enforced targeting of Crn7 to TGN bypasses the requirement of K33-ubiquitination for TGN-pool F-actin assembly and post-Golgi trafficking. Our study reveals a role of KLHL20-mediated K33-ubiquitination of Crn7 in post-Golgi transport and identifies a cellular recognition mechanism for this ubiquitin chain type. |
| Links |
PubMed Online version:10.1016/j.molcel.2014.03.035 |
| Keywords |
Actins/genetics; Actins/metabolism; Adaptor Proteins, Vesicular Transport/genetics; Adaptor Proteins, Vesicular Transport/metabolism; Animals; COS Cells; Carrier Proteins/genetics; Carrier Proteins/metabolism; Cell Line; Cercopithecus aethiops; Cullin Proteins/genetics; Cullin Proteins/metabolism; Golgi Apparatus/metabolism; HEK293 Cells; HeLa Cells; Humans; Lysine/metabolism; Microfilament Proteins/genetics; Microfilament Proteins/metabolism; Protein Transport; Ubiquitin-Protein Ligases/genetics; Ubiquitin-Protein Ligases/metabolism; Ubiquitination; trans-Golgi Network/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P57737 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:CORO7 |
enables |
GO:0003779: actin binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:CORO7 |
located_in |
GO:0005802: trans-Golgi network |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:CORO7 |
involved_in |
GO:0030041: actin filament polymerization |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:CORO7 |
involved_in |
GO:0006895: Golgi to endosome transport |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:CORO7 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9Y2M5 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:CORO7 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P42566 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:ZRAN1 |
involved_in |
GO:1990168: protein K33-linked deubiquitination |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
part_of |
GO:0031463: Cul3-RING ubiquitin ligase complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
located_in |
GO:0005802: trans-Golgi network |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
involved_in |
GO:1990390: protein K33-linked ubiquitination |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
contributes_to |
GO:0004842: ubiquitin-protein transferase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
involved_in |
GO:0006895: Golgi to endosome transport |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:KLH20 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P57737 |
F |
Seeded From UniProt |
complete | |
|
enables |
GO:0031593: polyubiquitin modification-dependent protein binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006895: Golgi to endosome transport |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0031463: Cul3-RING ubiquitin ligase complex |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
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