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PMID:24684932

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Citation

Phillips, CM, Montgomery, BE, Breen, PC, Roovers, EF, Rim, YS, Ohsumi, TK, Newman, MA, van Wolfswinkel, JC, Ketting, RF, Ruvkun, G and Montgomery, TA (2014) MUT-14 and SMUT-1 DEAD box RNA helicases have overlapping roles in germline RNAi and endogenous siRNA formation. Curr. Biol. 24:839-44

Abstract

More than 2,000 C. elegans genes are targeted for RNA silencing by the mutator complex, a specialized small interfering RNA (siRNA) amplification module which is nucleated by the Q/N-rich protein MUT-16. The mutator complex localizes to Mutator foci adjacent to P granules at the nuclear periphery in germ cells. Here, we show that the DEAD box RNA helicase smut-1 functions redundantly in the mutator pathway with its paralog mut-14 during RNAi. Mutations in both smut-1 and mut-14 also cause widespread loss of endogenous siRNAs. The targets of mut-14 and smut-1 largely overlap with the targets of other mutator class genes; however, the mut-14 smut-1 double mutant and the mut-16 mutant display the most dramatic depletion of siRNAs, suggesting that they act at a similarly early step in siRNA formation. mut-14 and smut-1 are predominantly expressed in the germline and, unlike other mutator class genes, are specifically required for RNAi targeting germline genes. A catalytically inactive, dominant-negative missense mutant of MUT-14 is RNAi defective in vivo; however, mutator complexes containing the mutant protein retain the ability to synthesize siRNAs in vitro. The results point to a role for mut-14 and smut-1 in initiating siRNA amplification in germ cell Mutator foci, possibly through the recruitment or retention of target mRNAs.

Links

PubMed PMC4010136 Online version:10.1016/j.cub.2014.02.060

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

CAEEL:G5ECM1

enables

GO:0003968: RNA-directed 5'-3' RNA polymerase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

CAEEL:O62011

is_active_in

GO:1990633: mutator focus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(WBbt:0005784)

Seeded From UniProt

complete

CAEEL:MUT7

located_in

GO:0005829: cytosol

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(WBbt:0008378)

Seeded From UniProt

complete

CAEEL:Q17978

involved_in

GO:0016246: RNA interference

ECO:0000316: genetic interaction evidence used in manual assertion

WB:WBGene00021391

P

  • occurs_in:(WBbt:0005784)

Seeded From UniProt

complete

CAEEL:Q17978

is_active_in

GO:1990633: mutator focus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(WBbt:0005784)

Seeded From UniProt

complete

CAEEL:O62011

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

CAEEL:O62011

part_of

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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