GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:24571519

From GONUTS
Jump to: navigation, search
Citation

Lee, E, Lucas, JR and Sack, FD (2014) Deep functional redundancy between FAMA and FOUR LIPS in stomatal development. Plant J. 78:555-65

Abstract

Functional redundancy arises between gene paralogs as well as non-homologous genes that play a common role at a shared node. The bHLH transcription factor FAMA, along with the paralogous MYB genes, FOUR LIPS (FLP) and MYB88 all ensure that Arabidopsis stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity. Consistent with this function, FLP and FAMA exhibit the same expression pattern in which both translational GFP fusions emit fluorescence just before and after symmetric division; however, FAMA but not FLP is required to confer GC fate. Strikingly, swapping the genes and promoters of the FLP and FAMA genes results in the reciprocal complementation of respective loss-of-function mutants. Thus, an FLP transgene can restore GC fate to a fama mutant background. FAMA, FLP and the FLP paralog MYB88 were previously shown to influence higher order functions in stomatal development, including maintaining and stabilizing stomatal fate. Here we show that these overlapping functions are likely to also involve interactions between FLP and FAMA with the RETINOBLASTOMA-RELATED (RBR) protein.

Links

PubMed Online version:10.1111/tpj.12489

Keywords

Arabidopsis/cytology; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Basic Helix-Loop-Helix Transcription Factors/genetics; Basic Helix-Loop-Helix Transcription Factors/metabolism; Cell Division; Gene Expression Regulation, Plant; Genetic Complementation Test; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Microscopy, Fluorescence; Models, Biological; Mutation; Phenotype; Plant Stomata/cytology; Plant Stomata/metabolism; Plants, Genetically Modified; Protein Binding; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors/genetics; Transcription Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:MYB88

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LKZ3

F

Seeded From UniProt

complete

ARATH:FAMA

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LKZ3

F

Seeded From UniProt

complete

ARATH:MY124

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9LKZ3

F

Seeded From UniProt

complete

ARATH:MY124

involved_in

GO:0010444: guard mother cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:RBR1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q56YJ8

F

Seeded From UniProt

complete

ARATH:RBR1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:F4IRB4

F

Seeded From UniProt

complete

ARATH:RBR1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q94FL6

F

Seeded From UniProt

complete

ARATH:FAMA

involved_in

GO:0010444: guard mother cell differentiation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.