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PMID:2439522

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Citation

Womer, RB, Frick, K, Mitchell, CD, Ross, AH, Bishayee, S and Scher, CD (1987) PDGF induces c-myc mRNA expression in MG-63 human osteosarcoma cells but does not stimulate cell replication. J. Cell. Physiol. 132:65-72

Abstract

The platelet-derived growth factor (PDGF) modulated growth response of the MG-63 human osteosarcoma cell line, which neither expresses c-sis mRNA nor secretes a PDGF analogue, was characterized. Scatchard analysis demonstrated that the MG-63 cells have 23,000 receptors per cell with a Kd of 5 X 10(-11) M. The receptor became phosphorylated, in a PDGF concentration-dependent manner, when 32P-orthophosphate-labeled cells were treated with PDGF for 3 h at 4 degrees C. The phosphorylated receptor was identified by autoradiography and gel electrophoresis after isolation of the 32P-labeled receptor using a solid-phase monoclonal antibody directed against phosphotyrosine. Binding of the receptor to the antibody was inhibited by 5 mM phenyl phosphate, further suggesting that PDGF stimulated tyrosine-specific receptor autophosphorylation. In addition, treatment of MG-63 cells with PDGF for 3 h at 37 degrees C induced a 7.5-fold increase in c-myc mRNA accumulation as analyzed on Northern gels. However, MG-63 cells grew equally well in either serum-(which contains PDGF) or plasma-(which does not) supplemented medium. Furthermore, PDGF did not stimulate DNA synthesis in growth arrested MG-63 cells, nor did it potentiate DNA synthesis modulated by somatomedin C. Thus MG-63 cells are a naturally occurring cell variant in which PDGF stimulates c-myc expression but does not modulate mitogenesis.

Links

PubMed Online version:10.1002/jcp.1041320109

Keywords

Animals; Cell Division/drug effects; Cell Line; DNA Replication/drug effects; Humans; Insulin-Like Growth Factor I/pharmacology; Mice; Mice, Inbred BALB C; Mitosis; Oncogenes; Osteosarcoma/genetics; Phosphorylation; Phosphotyrosine; Platelet-Derived Growth Factor/pharmacology; RNA, Messenger/metabolism; Receptors, Cell Surface/metabolism; Receptors, Platelet-Derived Growth Factor; Tyrosine/analogs & derivatives; Tyrosine/analysis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:PDGFB

enables

GO:0005161: platelet-derived growth factor receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PDGFB

involved_in

GO:0048146: positive regulation of fibroblast proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PDGFB

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PDGFB

involved_in

GO:0048008: platelet-derived growth factor receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PDGFA

enables

GO:0005161: platelet-derived growth factor receptor binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:PDGFA

involved_in

GO:0008284: positive regulation of cell population proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PDGFA

involved_in

GO:0048008: platelet-derived growth factor receptor signaling pathway

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:PDGFA

involved_in

GO:0048146: positive regulation of fibroblast proliferation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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