PMID:24265704

Citation	Zhang, C, Hong, Z, Ma, W, Ma, D, Qian, Y, Xie, W, Tie, F and Fang, M (2013) Drosophila UTX coordinates with p53 to regulate ku80 expression in response to DNA damage. PLoS ONE 8:e78652
Abstract	UTX is known as a general factor that activates gene transcription during development. Here, we demonstrate an additional essential role of UTX in the DNA damage response, in which it upregulates the expression of ku80 in Drosophila, both in cultured cells and in third instar larvae. We further showed that UTX mediates the expression of ku80 by the demethylation of H3K27me3 at the ku80 promoter upon exposure to ionizing radiation (IR) in a p53-dependent manner. UTX interacts physically with p53, and both UTX and p53 are recruited to the ku80 promoter following IR exposure in an interdependent manner. In contrast, the loss of utx has little impact on the expression of ku70, mre11, hid and reaper, suggesting the specific regulation of ku80 expression by UTX. Thus, our findings further elucidate the molecular function of UTX.
Links	PubMed PMC3827076 Online version:10.1371/journal.pone.0078652
Keywords	Animals; Antigens, Nuclear/genetics; Base Sequence; Cell Survival/genetics; Cell Survival/radiation effects; DNA Damage; DNA Repair/genetics; DNA Repair/radiation effects; DNA-Binding Proteins/genetics; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/cytology; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; Drosophila melanogaster/radiation effects; Gene Expression Regulation/genetics; Gene Expression Regulation/radiation effects; Histones/metabolism; Methylation/radiation effects; Oxidoreductases, N-Demethylating/genetics; Oxidoreductases, N-Demethylating/metabolism; Promoter Regions, Genetic/genetics; Promoter Regions, Genetic/radiation effects; Tumor Suppressor Protein p53/metabolism; Up-Regulation/genetics; Up-Regulation/radiation effects
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:Q8MSM8	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9NGX6	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VL07	involved_in	GO:0071557: histone H3-K27 demethylation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VL07	enables	GO:0071558: histone demethylase activity (H3-K27 specific)	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
DROME:Q9VL07	enables	GO:1990841: promoter-specific chromatin binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
DROME:Q9VL07	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VL07	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9VL07	involved_in	GO:1990248: regulation of transcription from RNA polymerase II promoter in response to DNA damage	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:HID	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:RPR	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q8IMZ4	enables	GO:1990841: promoter-specific chromatin binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
DROME:Q8IMZ4	involved_in	GO:0071480: cellular response to gamma radiation	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q8IMZ4	involved_in	GO:1990248: regulation of transcription from RNA polymerase II promoter in response to DNA damage	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q8IMZ4	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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