PMID:24187085

Citation	Vargas-Bautista, C, Rahlwes, K and Straight, P' (2013) Bacterial Competition Reveals Differential Regulation of the pks Genes by Bacillus subtilis. J. Bacteriol. '
Abstract	Bacillus subtilis is adaptable to many environments in part due to its ability to produce a broad range of bioactive compounds. One such compound, bacillaene, is a linear polyketide/non-ribosomal peptide produced by B. subtilis. The pks genes encode the enzymatic megacomplex that synthesizes bacillaene. The majority of pks genes appear to be organized as a giant operon (> 74kb from pksC-pksR). In previous work, a deletion of the pks operon in B. subtilis was found to induce prodiginine production by Streptomyces coelicolor. Here, colonies of wild type B. subtilis formed a spreading population that induced prodiginine production from Streptomyces lividans, suggesting differential regulation of pks genes and thus bacillaene. While the parent colony showed widespread induction of pks expression among cells in the population, we found the spreading cells uniformly and transiently repressed the expression of the pks genes. To identify regulators that control pks genes, we first determined the pattern of pks gene expression in liquid culture. Next we identified mutations in regulatory genes that disrupted the wildtype pattern of pks gene expression. We found expression of the pks genes requires the master regulator of development, Spo0A, through its repression of AbrB, and the stationary-phase regulator, CodY. Deletions of degU, comA, and scoC had moderate effects, disrupting the timing and level of pks gene expression. The observed patterns of expression suggest that complex regulation of bacillaene and other antibiotics optimizes competitive fitness for B. subtilis.
Links	PubMed Online version:10.1128/JB.01022-13
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACIU:Q0PEX8	NOT	GO:1900732: regulation of polyketide biosynthetic process	ECO:0000315:		P		Figure S1	complete CACAO 9064
BACIU:Q5Y5R6		GO:1900734: positive regulation of polyketide biosynthetic process	ECO:0000315:		P		Figure 6	complete CACAO 9071

References

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