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PMID:24060997

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Citation

'Ramírez, CA, Dea-Ayuela, MA, Gutiérrez-Blázquez, MD, Bolas-Fernández, F, Requena, JM and Puerta, CJ (2013) Identification of proteins interacting with HSP70 mRNAs in Leishmania braziliensis. J Proteomics '

Abstract

HSP70 protein is involved in Leishmania differentiation, apoptosis, antimony-resistance and host-immune response. Therefore, this protein and the regulatory mechanisms of HSP70 gene expression are promising targets for therapeutic intervention against leishmaniasis. The regulation of mRNA expression in trypanosomatids operates mostly through the interaction of trans-acting proteins, and elements located in the untranslated regions of mRNAs. The aim of this work was to identify protein factors interacting specifically with the Leishmania braziliensis HSP70 mRNAs. Thus, the 5' UTR and the two types of 3' UTRs (UTR-I and UTR-II) from L. braziliensis HSP70 genes were used as baits in pull down assays using total protein extracts from parasites cultured at 26 or 35°C. The captured proteins were resolved by two-dimensional gel electrophoresis (2-D) and identified by mass spectrometry (MS) analysis. As a result, 52 different proteins were identified based on their binding to the L. braziliensis HSP70-mRNAs. As expected, several of the identified proteins were related to RNA metabolism (27%) and translation process (7%). In addition, five hypothetical conserved proteins having motifs related with RNA interaction were also identified (9.6%). Nevertheless, unexpected proteins, apparently unrelated to the mRNA expression, were also identified. The biological significance of these and others L.braziliensis detected proteins, including the HSP70 itself, are discussed.

Links

PubMed Online version:10.1016/j.jprot.2013.09.008

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

LEIBR:A4HMU1

GO:0004335: galactokinase activity

ECO:0000314:

F

Figure 1.

complete
CACAO 8822

LEIBR:A4HLU3

GO:0003978: UDP-glucose 4-epimerase activity

ECO:0000314:

F

Figure 1.

complete
CACAO 8823

LEIBR:A4H8V4

GO:0008023: transcription elongation factor complex

ECO:0000314:

C

Figure 1

complete
CACAO 8824

LEIBR:A4HAE9

GO:0005853: eukaryotic translation elongation factor 1 complex

ECO:0000314:

C

Figure 1.

complete
CACAO 8825

LEIBR:A4HGN5

GO:0005662: DNA replication factor A complex

ECO:0000314:

C

Figure 1.

complete
CACAO 8826

LEIBR:A4HMX7

GO:0004004: ATP-dependent RNA helicase activity

ECO:0000314:

F

Figure 1.

complete
CACAO 8827

LEIBR:A4HNM7

GO:0042556: eukaryotic elongation factor-2 kinase regulator activity

ECO:0000314:

F

Figure 1

complete
CACAO 8828

See also

References

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