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PMID:24022302

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Citation

Malini, E, Grossi, S, Deganuto, M, Rosano, C, Parini, R, Dominisini, S, Cariati, R, Zampieri, S, Bembi, B, Filocamo, M and Dardis, A (2014) Functional analysis of 11 novel GBA alleles. Eur. J. Hum. Genet. 22:511-6

Abstract

Gaucher disease is the most frequent lysosomal storage disorder due to the deficiency of the acid β-glucosidase, encoded by the GBA gene. In this study, we report the structural and functional characterization of 11 novel GBA alleles. Seven single missense alleles, P159S, N188I, E235K, P245T, W312S, S366R and W381C, and two alleles carrying in cis mutations, (N188S; G265R) and (E326K; D380N), were studied for enzyme activity in transiently transfected cells. All mutants were inactive except the P159S, which retained 15% of wild-type activity. To further characterize the alleles carrying two in cis mutations, we expressed constructs bearing singly each mutation. The presence of G265R or D380N mutations completely abolished enzyme activity, while N188S and E326K mutants retained 25 and 54% of wild-type activity, respectively. Two mutations, affecting the acceptor splice site of introns 5 (c.589-1G>A) and 9 (c.1389-1G>A), led to the synthesis of aberrant mRNA. Unpredictably, family studies showed that two alleles resulted from germline or 'de novo' mutations. These results strengthen the importance of performing a complete and accurate molecular analysis of the GBA gene in order to avoid misleading conclusions and provide a comprehensive functional analysis of new GBA mutations.

Links

PubMed PMC3953908 Online version:10.1038/ejhg.2013.182

Keywords

Alleles; Cell Line; Gaucher Disease/diagnosis; Gaucher Disease/genetics; Genetic Association Studies; Genotype; Glucosylceramidase/genetics; Humans; Mutation, Missense; Protein Conformation; RNA Splice Sites/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:GLCM

involved_in

GO:0006680: glucosylceramide catabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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