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PMID:24005864

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Citation

Tahara, N, Bessho, Y and Matsui, T (2013) Celf1 is required for formation of endoderm-derived organs in zebrafish. Int J Mol Sci 14:18009-23

Abstract

We recently reported that an RNA binding protein called Cugbp Elav-like family member 1 (Celf1) regulates somite symmetry and left-right patterning in zebrafish. In this report, we show additional roles of Celf1 in zebrafish organogenesis. When celf1 is knocked down by using an antisense morpholino oligonucleotides (MO), liver buds fail to form, and pancreas buds do not form a cluster, suggesting earlier defects in endoderm organogenesis. As expected, we found failures in endoderm cell growth and migration during gastrulation in embryos injected with celf1-MOs. RNA immunoprecipitation revealed that Celf1 binds to gata5 and cdc42 mRNAs which are known to be involved in cell growth and migration, respectively. Our results therefore suggest that Celf1 regulates proper organogenesis of endoderm-derived tissues by regulating the expression of such targets.

Links

PubMed PMC3794766 Online version:10.3390/ijms140918009

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:CELF1

involved_in

GO:0031016: pancreas development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2,ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0061031: endodermal digestive tract morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2,ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2,ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:1990402: embryonic liver development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2,ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

GO:0001889: liver development

ECO:0000315:

P

Figure 2 A-F illustrates deficiencies in mutants to correctly form the liver.

complete
CACAO 8914

DANRE:CELF1

GO:0061113: pancreas morphogenesis

ECO:0000315:

P

Figure 2 G-I illustrates deficiencies in pancreas morphogenesis.

complete
CACAO 8915

DANRE:CELF1

GO:0048588: developmental cell growth

ECO:0000315:

P

Figure 3 shows a decrease in endodermal cell growth with KD mutants.

complete
CACAO 8916

DANRE:CELF1

GO:0016477: cell migration

ECO:0000315:

P

Figure 4

complete
CACAO 8917

DANRE:CELF1

involved_in

GO:0048588: developmental cell growth

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0016477: cell migration

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0061113: pancreas morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0001889: liver development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:1990402: embryonic liver development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2
ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0061031: endodermal digestive tract morphogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2
ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0048565: digestive tract development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2
ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

DANRE:CELF1

enables

GO:0003729: mRNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:CELF1

involved_in

GO:0031016: pancreas development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-121102-2
ZFIN:ZDB-MRPHLNO-121102-3

P

Seeded From UniProt

complete

See also

References

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