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PMID:23980030

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Citation

Yamashita, A, Takayama, T, Iwata, R and Yamamoto, M (2013) A novel factor Iss10 regulates Mmi1-mediated selective elimination of meiotic transcripts. Nucleic Acids Res. 41:9680-7

Abstract

A number of meiosis-specific transcripts are selectively eliminated during the mitotic cell cycle in fission yeast. Mmi1, an RNA-binding protein, plays a crucial role in this selective elimination. Mmi1 recognizes a specific region, namely, the determinant of selective removal (DSR) on meiotic transcripts and induces nuclear exosome-mediated elimination. During meiosis, Mmi1 is sequestered by a chromosome-associated dot structure, Mei2 dot, allowing meiosis-specific transcripts to be stably expressed. Red1, a zinc-finger protein, is also known to participate in the Mmi1/DSR elimination system, although its molecular function has remained elusive. To uncover the detailed molecular mechanisms underlying the Mmi1/DSR elimination system, we sought to identify factors that interact genetically with Mmi1. Here, we show that one of the identified factors, Iss10, is involved in the Mmi1/DSR system by regulating the interaction between Mmi1 and Red1. In cells lacking Iss10, association of Red1 with Mmi1 is severely impaired, and target transcripts of Mmi1 are ectopically expressed in the mitotic cycle. During meiosis, Iss10 is downregulated, resulting in dissociation of Red1 from Mmi1 and subsequent suppression of Mmi1 activity.

Links

PubMed PMC3834831 Online version:10.1093/nar/gkt763

Keywords

Cell Cycle Proteins/analysis; Cell Cycle Proteins/genetics; Cell Cycle Proteins/physiology; Gene Expression Regulation, Fungal; Meiosis/genetics; RNA, Messenger/metabolism; Schizosaccharomyces/chemistry; Schizosaccharomyces/genetics; Schizosaccharomyces/metabolism; Schizosaccharomyces pombe Proteins/analysis; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism; Schizosaccharomyces pombe Proteins/physiology; mRNA Cleavage and Polyadenylation Factors/analysis; mRNA Cleavage and Polyadenylation Factors/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:YFPE

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPAC1006.03c

F

Seeded From UniProt

complete

SCHPO:YFPE

involved_in

GO:0033621: nuclear-transcribed mRNA catabolic process, meiosis-specific transcripts

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:YFPE

is_active_in

GO:1990251: nuclear exosome focus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:MMI1

is_active_in

GO:1990251: nuclear exosome focus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:MMI1

part_of

GO:0033620: Mei2 nuclear dot complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:RED1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

PomBase:SPAC7D4.14c

F

Seeded From UniProt

complete

SCHPO:RED1

involved_in

GO:0033621: nuclear-transcribed mRNA catabolic process, meiosis-specific transcripts

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:RED1

is_active_in

GO:1990251: nuclear exosome focus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

SCHPO:YLP3

involved_in

GO:0033621: nuclear-transcribed mRNA catabolic process, meiosis-specific transcripts

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:YLP3

part_of

GO:1990251: Mmi1 nuclear focus

ECO:0000314: direct assay evidence used in manual assertion

C

exists_during:(GO:0000278)

Seeded From UniProt

complete

Notes

See also

References

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