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PMID:23926254

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Citation

Kaneko-Goto, T, Sato, Y, Katada, S, Kinameri, E, Yoshihara, S, Nishiyori, A, Kimura, M, Fujita, H, Touhara, K, Reed, RR and Yoshihara, Y (2013) Goofy coordinates the acuity of olfactory signaling. J. Neurosci. 33:12987-96, 12996a

Abstract

The basic scheme of odor perception and signaling from olfactory cilia to the brain is well understood. However, factors that affect olfactory acuity of an animal, the threshold sensitivity to odorants, are less well studied. Using signal sequence trap screening of a mouse olfactory epithelium cDNA library, we identified a novel molecule, Goofy, that is essential for olfactory acuity in mice. Goofy encodes an integral membrane protein with specific expression in the olfactory and vomeronasal sensory neurons and predominant localization to the Golgi compartment. Goofy-deficient mice display aberrant olfactory phenotypes, including the impaired trafficking of adenylyl cyclase III, stunted olfactory cilia, and a higher threshold for physiological and behavioral responses to odorants. In addition, the expression of dominant-negative form of cAMP-dependent protein kinase results in shortening of olfactory cilia, implying a possible mechanistic link between cAMP and ciliogenesis in the olfactory sensory neurons. These results demonstrate that Goofy plays an important role in establishing the acuity of olfactory sensory signaling.

Links

PubMed Online version:10.1523/JNEUROSCI.4948-12.2013

Keywords

Adenylyl Cyclases/metabolism; Animals; Cloning, Molecular; Cyclic AMP-Dependent Protein Kinases/genetics; Cyclic Nucleotide-Gated Cation Channels/genetics; Cyclic Nucleotide-Gated Cation Channels/metabolism; Evoked Potentials/genetics; GTP-Binding Proteins/deficiency; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; Gene Expression Regulation/genetics; Luminescent Proteins/genetics; Male; Mice; Mice, Inbred C57BL; Odorants; Olfactory Marker Protein/genetics; Olfactory Marker Protein/metabolism; Olfactory Pathways/anatomy & histology; Olfactory Pathways/metabolism; Olfactory Receptor Neurons/physiology; RNA, Messenger; Receptors, Odorant/genetics; Receptors, Odorant/metabolism; Sequence Analysis; Signal Transduction/genetics; Signal Transduction/physiology; Trans-Activators/genetics; Trans-Activators/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:GFY

located_in

GO:0030173: integral component of Golgi membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

MOUSE:GFY

involved_in

GO:0007608: sensory perception of smell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:GFY

acts_upstream_of_or_within

GO:0007608: sensory perception of smell

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:GFY

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)|part_of:(EMAPA:18444)
  • part_of:(CL:1001561)|part_of:(EMAPA:35764)

Seeded From UniProt

complete

MOUSE:GFY

acts_upstream_of_or_within

GO:0097499: protein localization to non-motile cilium

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

MOUSE:GFY

acts_upstream_of_or_within

GO:1905515: non-motile cilium assembly

ECO:0000315: mutant phenotype evidence used in manual assertion

P

  • occurs_in:(EMAPA:17606)
  • occurs_in:(CL:0000207)

Seeded From UniProt

complete

MOUSE:LAMP1

located_in

GO:0005764: lysosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:BIP

located_in

GO:0005783: endoplasmic reticulum

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:MCL1

located_in

GO:0005739: mitochondrion

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:EEA1

located_in

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:GOGA2

located_in

GO:0005801: cis-Golgi network

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:EEA1

part_of

GO:0005769: early endosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:LAMP1

part_of

GO:0005764: lysosome

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(EMAPA:17606)
  • part_of:(CL:0000207)

Seeded From UniProt

complete

MOUSE:GOGA2

part_of

GO:0005801: cis-Golgi network

ECO:0000314: direct assay evidence used in manual assertion

C

  • part_of:(CL:0000207)
  • part_of:(EMAPA:17606)

Seeded From UniProt

complete

Notes

See also

References

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