GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:23722546

From GONUTS
Jump to: navigation, search
Citation

Levi, L, Lobo, G, Doud, MK, von Lintig, J, Seachrist, D, Tochtrop, GP and Noy, N (2013) Genetic ablation of the fatty acid-binding protein FABP5 suppresses HER2-induced mammary tumorigenesis. Cancer Res. 73:4770-80

Abstract

The fatty acid-binding protein FABP5 shuttles ligands from the cytosol to the nuclear receptor PPARβ/δ (encoded for by Pparδ), thereby enhancing the transcriptional activity of the receptor. This FABP5/PPARδ pathway is critical for induction of proliferation of breast carcinoma cells by activated epidermal growth factor receptor (EGFR). In this study, we show that FABP5 is highly upregulated in human breast cancers and we provide genetic evidence of the pathophysiologic significance of FABP5 in mammary tumorigenesis. Ectopic expression of FABP5 was found to be oncogenic in 3T3 fibroblasts where it augmented the ability of PPARδ to enhance cell proliferation, migration, and invasion. To determine whether FABP5 is essential for EGFR-induced mammary tumor growth, we interbred FABP5-null mice with MMTV-ErbB2/HER2 oncomice, which spontaneously develop mammary tumors. FABP5 ablation relieved activation of EGFR downstream effector signals, decreased expression of PPARδ target genes that drive cell proliferation, and suppressed mammary tumor development. Our findings establish that FABP5 is critical for mammary tumor development, rationalizing the development of FABP5 inhibitors as novel anticarcinogenic drugs.

Links

PubMed PMC4082958 Online version:10.1158/0008-5472.CAN-13-0384

Keywords

Animals; Cell Movement/physiology; Fatty Acid-Binding Proteins/genetics; Fatty Acid-Binding Proteins/metabolism; Female; Humans; Immunoblotting; Immunohistochemistry; Mammary Neoplasms, Experimental/genetics; Mammary Neoplasms, Experimental/metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Neoplasm Proteins/genetics; Neoplasm Proteins/metabolism; PPAR delta/metabolism; Real-Time Polymerase Chain Reaction; Receptor, ErbB-2/genetics; Receptor, ErbB-2/metabolism; Signal Transduction/physiology; Up-Regulation; Zebrafish

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:FABP5

GO:0048144: fibroblast proliferation

ECO:0000315:

P

MOUSE FABP5 // 3T3 clones that overexpress different levels of FABP5 were produced (Fig. 2F) and their rates of proliferation were measured using MTT assay. Overexpression of FABP5 facilitated the proliferation of 3T3 cells and did so in a dose-responsive fashion (Fig. 2G).

complete
CACAO 12328

Notes

See also

References

See Help:References for how to manage references in GONUTS.