PMID:23686420

Citation	'Kelly, AA, van Erp, H, Quettier, AL, Shaw, E, Menard, G, Kurup, S and Eastmond, PJ (2013) The SUGAR-DEPENDENT1 lipase limits triacylglycerol accumulation in vegetative tissues of Arabidopsis. Plant Physiol. '
Abstract	There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step-change in oil yield. Here we show that disruption of TAG hydrolysis in the Arabidopsis thaliana lipase mutant sugar-dependent1 (sdp1) leads to a substantial accumulation of TAG in roots and stems, but comparatively much lower TAG accumulation in leaves. TAG content in sdp1 roots increases with the age of the plant and can reach more than 1% of dry weight (DW) at maturity; a 50-fold increase over wild type. TAG accumulation in sdp1 roots requires both ACYL-CoA:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and PHOSPHATIDYLCHOLINE:DIACYLGLYCEROL ACYLTRANSFERASE1 and can also be strongly stimulated by the provision of exogenous sugar. In transgenic plants constitutively co-expressing WRINKLED1 and DGAT1, sdp1 also doubles the accumulation of TAG in roots, stems and leaves, with levels ranging from 5 to 8% of DW. Finally provision of 3% (w/v) exogenous sucrose can further boost root TAG content in these transgenic plants to 17% of DW. This level of TAG is similar to seed tissues in many plant species and establishes the efficacy of an engineering strategy to produce oil in vegetative tissues that involves simultaneous manipulation of carbohydrate supply, fatty acid synthesis, TAG synthesis and also TAG breakdown.
Links	PubMed Online version:10.1104/pp.113.219840
Keywords
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:SDP1		GO:0019433: triglyceride catabolic process	ECO:0000315:		P		Figure 2	complete CACAO 8438
ARATH:SDP1	involved_in	GO:0019433: triglyceride catabolic process	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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