GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:2360047

From GONUTS
Jump to: navigation, search
Citation

Oettinger, MA, Schatz, DG, Gorka, C and Baltimore, D (1990) RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science 248:1517-23

Abstract

The vast repertoire of immunoglobulins and T cell receptors is generated, in part, by V(D)J recombination, a series of genomic rearrangements that occur specifically in developing lymphocytes. The recombination activating gene, RAG-1, which is a gene expressed exclusively in maturing lymphoid cells, was previously isolated. RAG-1 inefficiently induced V(D)J recombinase activity when transfected into fibroblasts, but cotransfection with an adjacent gene, RAG-2, has resulted in at least a 1000-fold increase in the frequency of recombination. The 2.1-kilobase RAG-2 complementary DNA encodes a putative protein of 527 amino acids whose sequence is unrelated to that of RAG-1. Like RAG-1, RAG-2 is conserved between species that carry out V(D)J recombination, and its expression pattern correlates precisely with that of V(D)J recombinase activity. In addition to being located just 8 kilobases apart, these convergently transcribed genes are unusual in that most, if not all, of their coding and 3' untranslated sequences are contained in single exons. RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction.

Links

PubMed

Keywords

Amino Acid Sequence; Animals; Base Sequence; Biological Evolution; Cattle; Cell Line; Chickens; Cricetinae; DNA/genetics; DNA Nucleotidyltransferases/genetics; DNA-Binding Proteins; Dogs; Female; Gene Rearrangement, B-Lymphocyte; Gene Rearrangement, T-Lymphocyte; Homeodomain Proteins; Humans; Male; Mice; Molecular Sequence Data; Multigene Family; Nuclear Proteins; Nucleic Acid Hybridization; Opossums; Proteins/genetics; Rabbits; Recombination, Genetic/genetics; Restriction Mapping; Transfection; Turtles; VDJ Recombinases

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

MOUSE:RAG1

acts_upstream_of_or_within

GO:0033151: V(D)J recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97849

P

  • occurs_in:(CL:0000057)

Seeded From UniProt

complete

MOUSE:RAG2

acts_upstream_of_or_within

GO:0033151: V(D)J recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97848

P

  • occurs_in:(CL:0000057)

Seeded From UniProt

complete

MOUSE:RAG1

acts_upstream_of_or_within

GO:0033151: V(D)J recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97849

P

occurs_in:(CL:0000057)

Seeded From UniProt

complete

MOUSE:RAG2

acts_upstream_of_or_within

GO:0033151: V(D)J recombination

ECO:0000316: genetic interaction evidence used in manual assertion

MGI:MGI:97848

P

occurs_in:(CL:0000057)

Seeded From UniProt

complete

See also

References

See Help:References for how to manage references in GONUTS.