PMID:23583758

Citation	Schertel, C, Huang, D, Björklund, M, Bischof, J, Yin, D, Li, R, Wu, Y, Zeng, R, Wu, J, Taipale, J, Song, H and Basler, K (2013) Systematic screening of a Drosophila ORF library in vivo uncovers Wnt/Wg pathway components. Dev. Cell 25:207-19
Abstract	We created a site-directed UAS-ORF library of 655 growth-regulating genes in Drosophila. This library represents a large collection of genes regulating cell cycle, cell size, and proliferation and will be a valuable resource for studying growth regulation in vivo. By using misexpression of genes, we prevent problems arising from genetic redundancy and can uncover novel gene functions. To validate the usefulness of this library, we screened for Wingless (Wg) pathway components. We used a combination of experimental and bioinformatic approaches to predict candidates and identified three serine/threonine kinases as regulators of Wg signaling. We show that one of these, Nek2, optimizes pathway response by direct phosphorylation of Dishevelled. In addition, we describe functional relations for roughly 5% of all Drosophila genes and identify a large number of genes that regulate cell size, proliferation, and final organ size upon misexpression.
Links	PubMed Online version:10.1016/j.devcel.2013.02.019
Keywords	Adaptor Proteins, Signal Transducing/genetics; Adaptor Proteins, Signal Transducing/metabolism; Animals; Computational Biology; Dishevelled Proteins; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/growth & development; Drosophila melanogaster/metabolism; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Gene Expression Regulation, Developmental; Gene Library; Immunoenzyme Techniques; Luciferases/metabolism; Open Reading Frames/genetics; Phosphoproteins/genetics; Phosphoproteins/metabolism; Phosphorylation; Signal Transduction; Wnt Proteins/genetics; Wnt Proteins/metabolism; Wnt1 Protein/genetics; Wnt1 Protein/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
DROME:O62602	involved_in	GO:0090090: negative regulation of canonical Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:DCO	involved_in	GO:0030177: positive regulation of Wnt signaling pathway	ECO:0000316: genetic interaction evidence used in manual assertion	FB:FBgn0029970	P		Seeded From UniProt	complete
DROME:Q9W3N8	enables	GO:0004674: protein serine/threonine kinase activity	ECO:0000314: direct assay evidence used in manual assertion		F	has_input:(FB:FBgn0000499) part_of:(GO:0090263)	Seeded From UniProt	complete
DROME:Q9W3N8	involved_in	GO:0006468: protein phosphorylation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
DROME:Q9W3N8	involved_in	GO:0090263: positive regulation of canonical Wnt signaling pathway	ECO:0000316: genetic interaction evidence used in manual assertion	FB:FBgn0284084	P		Seeded From UniProt	complete
DROME:Q9W3N8	involved_in	GO:0090263: positive regulation of canonical Wnt signaling pathway	ECO:0000316: genetic interaction evidence used in manual assertion	FB:FBgn0000499	P		Seeded From UniProt	complete
DROME:DYRK2	involved_in	GO:0030177: positive regulation of Wnt signaling pathway	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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