PMID:23437850

Citation	Kim, HS, Abbasi, N and Choi, SB (2013) Bruno-like proteins modulate flowering time via 3' UTR-dependent decay of SOC1 mRNA. New Phytol. 198:747-56
Abstract	The Bruno RNA-binding protein (RBP) has been shown to initially repress the translation of oskar mRNA during Drosophila oogenesis and later to be involved in a broad range of RNA regulation. Here, we show that homologous constitutive overexpression of each of two Arabidopsis thaliana Bruno-like genes, AtBRN1 and AtBRN2, delayed the flowering time, while the atbrn1 atbrn2-3 double mutant flowered early and exhibited increased expression of APETALA1 (AP1) and LEAFY (LFY) transcripts. Crossing of 35S::AtBRNs with SOC1 101-D plants demonstrated that 35S::AtBRNs suppress an early-flowering phenotype of SOC1 101-D in which the coding sequence (CDS) with the 3' UTR of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1) gene is overexpressed. However, this early-flowering phenotype by SOC1 overexpression was maintained in the plants coexpressing 35S::AtBRNs and 35S::SOC1 without the 3' UTR (-3' UTR). Using yeast three-hybrid, electrophoretic mobility shift, RNA immunoprecipitation, and protoplast transient assays, we found that AtBRNs bind to the 3' UTR of SOC1 RNA and participate in mRNA decay, which was mediated by the distal region of the SOC1 3' UTR. Overall, AtBRNs repress SOC1 activity in a 3' UTR-dependent manner, thereby controlling the flowering time in Arabidopsis.
Links	PubMed Online version:10.1111/nph.12181
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:BRN2L	located_in	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
ARATH:BRN1L	enables	GO:0003729: mRNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
ARATH:BRN1L	involved_in	GO:0006402: mRNA catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:BRN1L	involved_in	GO:2000028: regulation of photoperiodism, flowering	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:BRN2L		GO:0048573: photoperiodism, flowering	ECO:0000315:		P		Figure 2	complete CACAO 8188
ARATH:BRN2L	involved_in	GO:0048573: photoperiodism, flowering	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:BRN2L	involved_in	GO:0006402: mRNA catabolic process	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:BRN2L	involved_in	GO:2000028: regulation of photoperiodism, flowering	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
ARATH:BRN2L	part_of	GO:0005737: cytoplasm	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
ARATH:BRN2L	enables	GO:0003729: mRNA binding	ECO:0000314: direct assay evidence used in manual assertion		F		Seeded From UniProt	complete

References

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