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PMID:23426364

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Citation

Nonaka, S, Nagaosa, K, Mori, T, Shiratsuchi, A and Nakanishi, Y (2013) Integrin αPS3/βν-mediated phagocytosis of apoptotic cells and bacteria in Drosophila. J. Biol. Chem. 288:10374-80

Abstract

Integrins exert a variety of cellular functions as heterodimers of two transmembrane subunits named α and β. Integrin βν, a β-subunit of Drosophila integrin, is involved in the phagocytosis of apoptotic cells and bacteria. Here, we searched for an α-subunit that forms a complex and cooperates with βν. Examinations of RNAi-treated animals suggested that αPS3, but not any of four other α-subunits, is required for the effective phagocytosis of apoptotic cells in Drosophila embryos. The mutation of αPS3-encoding scb, deficiency, insertion of P-element, or alteration of nucleotide sequences, brought about a reduction in the level of phagocytosis. The defect in phagocytosis by deficiency was reverted by the forced expression of scb. Furthermore, flies in which the expression of both αPS3 and βν was inhibited by RNAi showed a level of phagocytosis almost equal to that observed in flies with RNAi for either subunit alone. A loss of αPS3 also decreased the activity of larval hemocytes in the phagocytosis of Staphylococcus aureus. Finally, a co-immunoprecipitation analysis using a Drosophila cell line treated with a chemical cross-linker suggested a physical association between αPS3 and βν. These results collectively indicated that integrin αPS3/βν serves as a receptor in the phagocytosis of apoptotic cells and bacteria by Drosophila phagocytes.

Links

PubMed PMC3624420 Online version:10.1074/jbc.M113.451427

Keywords

Animals; Apoptosis/physiology; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster; Embryo, Nonmammalian/cytology; Embryo, Nonmammalian/metabolism; Hemocytes/cytology; Hemocytes/metabolism; Integrin alpha Chains/genetics; Integrin alpha Chains/metabolism; Integrin beta Chains/genetics; Integrin beta Chains/metabolism; Mutation; Phagocytosis/physiology; Staphylococcus aureus

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:ITA3

located_in

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITBN

located_in

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITBN

involved_in

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ITBN

enables

GO:0046982: protein heterodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0286785

F

Seeded From UniProt

complete

DROME:ITBN

part_of

GO:0008305: integrin complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITBN

involved_in

GO:0043277: apoptotic cell clearance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ITA3

part_of

GO:0016021: integral component of membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITA3

part_of

GO:0008305: integrin complex

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:ITA3

involved_in

GO:0043277: apoptotic cell clearance

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ITA3

involved_in

GO:0006909: phagocytosis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:ITA3

enables

GO:0046982: protein heterodimerization activity

ECO:0000353: physical interaction evidence used in manual assertion

FB:FBgn0010395

F

Seeded From UniProt

complete

Notes

See also

References

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