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PMID:23394836

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Citation

Xu, Y, Wang, Y, Stroud, H, Gu, X, Sun, B, Gan, ES, Ng, KH, Jacobsen, SE, He, Y and Ito, T (2013) A matrix protein silences transposons and repeats through interaction with retinoblastoma-associated proteins. Curr. Biol. 23:345-50

Abstract

Epigenetic regulation helps to maintain genomic integrity by suppressing transposable elements (TEs) and also controls key developmental processes, such as flowering time. To prevent TEs from causing rearrangements and mutations, TE and TE-like repetitive DNA sequences are usually methylated, whereas histones are hypoacetylated and methylated on specific residues (e.g., H3 lysine 9 dimethylation [H3K9me2]). TEs and repeats can also attenuate gene expression. However, how various histone modifiers are recruited to target loci is not well understood. Here we show that knockdown of the nuclear matrix protein with AT-hook DNA binding motifs TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK) in Arabidopsis Landsberg erecta results in robust activation of various TEs, the TE-like repeat-containing floral repressor genes FLOWERING LOCUS C (FLC) and FWA. This derepression is associated with chromatin conformational changes, increased histone acetylation, reduced H3K9me2, and even TE transposition. TEK directly binds to an FLC-repressive regulatory region and the silencing repeats of FWA and associates with Arabidopsis homologs of the Retinoblastoma-associated protein 46/48, FVE and MSI5, which mediate histone deacetylation. We propose that the nuclear matrix protein TEK acts in the maintenance of genome integrity by silencing TE and repeat-containing genes.

Links

PubMed Online version:10.1016/j.cub.2013.01.030

Keywords

Arabidopsis/genetics; Chromatin/genetics; Chromatin/metabolism; DNA Methylation; DNA Transposable Elements/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Epigenesis, Genetic; Gene Expression Regulation, Plant; Nuclear Matrix-Associated Proteins/genetics; RNA Interference; RNA, Small Interfering; Retinoblastoma Binding Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:MSI4

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SJG4

F

Seeded From UniProt

complete

ARATH:AHL16

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SU78

F

Seeded From UniProt

complete

ARATH:AHL16

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:O22607

F

Seeded From UniProt

complete

ARATH:AHL16

acts_upstream_of_or_within

GO:0010529: negative regulation of transposition

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AHL16

acts_upstream_of_or_within

GO:0010228: vegetative to reproductive phase transition of meristem

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AHL16

enables

GO:0031490: chromatin DNA binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

ARATH:AHL16

acts_upstream_of_or_within

GO:1900111: positive regulation of histone H3-K9 dimethylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:AHL16

acts_upstream_of_or_within

GO:0035067: negative regulation of histone acetylation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:MSI5

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9SJG4

F

Seeded From UniProt

complete

Notes

See also

References

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