GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:23374346

From GONUTS
Jump to: navigation, search
Citation

Mugoni, V, Postel, R, Catanzaro, V, De Luca, E, Turco, E, Digilio, G, Silengo, L, Murphy, MP, Medana, C, Stainier, DY, Bakkers, J and Santoro, MM (2013) Ubiad1 is an antioxidant enzyme that regulates eNOS activity by CoQ10 synthesis. Cell 152:504-18

Abstract

Protection against oxidative damage caused by excessive reactive oxygen species (ROS) by an antioxidant network is essential for the health of tissues, especially in the cardiovascular system. Here, we identified a gene with important antioxidant features by analyzing a null allele of zebrafish ubiad1, called barolo (bar). bar mutants show specific cardiovascular failure due to oxidative stress and ROS-mediated cellular damage. Human UBIAD1 is a nonmitochondrial prenyltransferase that synthesizes CoQ10 in the Golgi membrane compartment. Loss of UBIAD1 reduces the cytosolic pool of the antioxidant CoQ10 and leads to ROS-mediated lipid peroxidation in vascular cells. Surprisingly, inhibition of eNOS prevents Ubiad1-dependent cardiovascular oxidative damage, suggesting a crucial role for this enzyme and nonmitochondrial CoQ10 in NO signaling. These findings identify UBIAD1 as a nonmitochondrial CoQ10-forming enzyme with specific cardiovascular protective function via the modulation of eNOS activity.

Links

PubMed PMC3574195 Online version:10.1016/j.cell.2013.01.013

Keywords

Animals; Dimethylallyltranstransferase/genetics; Dimethylallyltranstransferase/metabolism; Endothelial Cells/metabolism; Golgi Apparatus/metabolism; Heart/embryology; Humans; Myocardium/cytology; Nitric Oxide Synthase Type III/metabolism; Reactive Oxygen Species/metabolism; Ubiquinone/analogs & derivatives; Ubiquinone/genetics; Zebrafish/embryology; Zebrafish/metabolism; Zebrafish Proteins/genetics; Zebrafish Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:UBIA1

enables

GO:0016209: antioxidant activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:UBIA1

located_in

GO:0030173: integral component of Golgi membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:UBIA1

enables

GO:0004659: prenyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0072359: circulatory system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-4

P

Seeded From UniProt

complete

DANRE:UBIA1

enables

GO:0004517: nitric-oxide synthase activity

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5,ZFIN:ZDB-MRPHLNO-100503-13

F

Seeded From UniProt

complete

DANRE:UBIA1

located_in

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0072359: circulatory system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-130327-5

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0072359: circulatory system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0072359: circulatory system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-4

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0071498: cellular response to fluid shear stress

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5,ZFIN:ZDB-MRPHLNO-100610-8

P

Seeded From UniProt

complete

DANRE:UBIA1

involved_in

GO:0071498: cellular response to fluid shear stress

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5,ZFIN:ZDB-MRPHLNO-060317-4

P

Seeded From UniProt

complete

HUMAN:UBIA1

enables

GO:0016209: antioxidant activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:UBIA1

located_in

GO:0030173: integral component of Golgi membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:UBIA1

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-4

P

Seeded From UniProt

complete

DANRE:E7FB98

part_of

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:E7FB98

enables

GO:0004517: nitric-oxide synthase activity

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5
ZFIN:ZDB-MRPHLNO-100503-13

F

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0071498: cellular response to fluid shear stress

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5
ZFIN:ZDB-MRPHLNO-060317-4

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0071498: cellular response to fluid shear stress

ECO:0000316: genetic interaction evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5
ZFIN:ZDB-MRPHLNO-100610-8

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0072358: cardiovascular system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-4

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0072358: cardiovascular system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-130327-5

P

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0072358: cardiovascular system development

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-MRPHLNO-130327-5

P

Seeded From UniProt

complete

DANRE:E7FB98

enables

GO:0004659: prenyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0006744: ubiquinone biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:E7FB98

enables

GO:0016209: antioxidant activity

ECO:0000315: mutant phenotype evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:E7FB98

part_of

GO:0030173: integral component of Golgi membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:E7FB98

involved_in

GO:0072358: cardiovascular system development

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

See Help:References for how to manage references in GONUTS.