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PMID:23354684
| Citation |
Kostrominova, TY and Brooks, SV (2013) Age-related changes in structure and extracellular matrix protein expression levels in rat tendons. Age (Dordr) 35:2203-14 |
|---|---|
| Abstract |
The musculoskeletal system (muscle-tendon-bone) demonstrates numerous age-related changes, with modifications in tendons the least well studied, although increased predisposition to tendinopathy and rupture have been reported. In order to gain insights into the basis of age-associated increase in tendon injuries, we compared Achilles and tibialis anterior tendons and myotendinous junctions (MTJs) from 3- to 5- and 22- to 25-month-old rats for underlying structure and composition. Significant decreases were observed by qRT-PCR for collagen I, III, and V mRNA expression in tendons of old rats, but immunostaining detected no apparent differences in collagen I and V expression on the protein level. Tendons of old compared with young rats had decreased mRNA expression levels of proteoglycan 4 (PRG4) and elastin (Eln), but no differences in the mRNA expression of connective tissue growth factor, TGF-beta 1, or stromal cell-derived factor 1. For PRG4, immunostaining showed good correlation with qRT-PCR results. This is the first study to show reductions in PRG4 in tendons and MTJs of old rats. Decreased PRG4 expression in tendons could result in increased tendon stiffness and may be associated with decreased activity in the elderly. The diminished collagen mRNA expression in combination with decreased PRG4 and Eln mRNA expression may be associated with increased risk of tendon injury with aging. |
| Links |
PubMed PMC3824999 Online version:10.1007/s11357-013-9514-2 |
| Keywords |
Achilles Tendon/cytology; Achilles Tendon/growth & development; Achilles Tendon/injuries; Achilles Tendon/metabolism; Aging; Animals; Disease Models, Animal; Extracellular Matrix Proteins/biosynthesis; Extracellular Matrix Proteins/genetics; Female; Gene Expression Regulation, Developmental; Immunohistochemistry; Male; RNA, Messenger/genetics; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Tendon Injuries/genetics; Tendon Injuries/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0007568: aging |
ECO:0000270: expression pattern evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
Notes
See also
References
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