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PMID:23345592

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Citation

Ng, MM, Dippold, HC, Buschman, MD, Noakes, CJ and Field, SJ (2013) GOLPH3L antagonizes GOLPH3 to determine Golgi morphology. Mol. Biol. Cell 24:796-808

Abstract

GOLPH3 is a phosphatidylinositol-4-phosphate (PI4P) effector that plays an important role in maintaining Golgi architecture and anterograde trafficking. GOLPH3 does so through its ability to link trans-Golgi membranes to F-actin via its interaction with myosin 18A (MYO18A). GOLPH3 also is known to be an oncogene commonly amplified in human cancers. GOLPH3L is a GOLPH3 paralogue found in all vertebrate genomes, although previously it was largely uncharacterized. Here we demonstrate that although GOLPH3 is ubiquitously expressed in mammalian cells, GOLPH3L is present in only a subset of tissues and cell types, particularly secretory tissues. We show that, like GOLPH3, GOLPH3L binds to PI4P, localizes to the Golgi as a consequence of its PI4P binding, and is required for efficient anterograde trafficking. Surprisingly, however, we find that perturbations of GOLPH3L expression produce effects on Golgi morphology that are opposite to those of GOLPH3 and MYO18A. GOLPH3L differs critically from GOLPH3 in that it is largely unable to bind to MYO18A. Our data demonstrate that despite their similarities, unexpectedly, GOLPH3L antagonizes GOLPH3/MYO18A at the Golgi.

Links

PubMed PMC3596250 Online version:10.1091/mbc.E12-07-0525

Keywords

3T3 Cells; Amino Acid Sequence; Animals; Cell Line; Glycosyltransferases/metabolism; Golgi Apparatus/metabolism; Golgi Apparatus/ultrastructure; HEK293 Cells; HeLa Cells; Humans; MCF-7 Cells; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mice; Myosins/genetics; Myosins/metabolism; Phosphatidylinositol Phosphates/metabolism; Phosphoproteins/metabolism; Protein Transport; RNA Interference; RNA, Small Interfering; Sequence Alignment; Signal Transduction

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:GLP3L

located_in

GO:0000139: Golgi membrane

ECO:0000305: curator inference used in manual assertion

GO:0070273

C

Seeded From UniProt

complete

HUMAN:GLP3L

located_in

GO:0032588: trans-Golgi network membrane

ECO:0000305: curator inference used in manual assertion

GO:0070273

C

Seeded From UniProt

complete

HUMAN:GLP3L

involved_in

GO:0007030: Golgi organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLP3L

involved_in

GO:0050714: positive regulation of protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GLP3L

enables

GO:0070273: phosphatidylinositol-4-phosphate binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:MY18A

involved_in

GO:0050714: positive regulation of protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GOLP3

involved_in

GO:0007030: Golgi organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GOLP3

involved_in

GO:0050714: positive regulation of protein secretion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:GOLP3

enables

GO:0070273: phosphatidylinositol-4-phosphate binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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