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PMID:23024348

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Citation

Chand, NS, Clatworthy, AE and Hung, DT (2012) The two-component sensor KinB acts as a phosphatase to regulate Pseudomonas aeruginosa Virulence. J. Bacteriol. 194:6537-47

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen that is capable of causing both acute and chronic infections. P. aeruginosa virulence is subject to sophisticated regulatory control by two-component systems that enable it to sense and respond to environmental stimuli. We recently reported that the two-component sensor KinB regulates virulence in acute P. aeruginosa infection. Furthermore, it regulates acute-virulence-associated phenotypes such as pyocyanin production, elastase production, and motility in a manner independent of its kinase activity. Here we show that KinB regulates virulence through the global sigma factor AlgU, which plays a key role in repressing P. aeruginosa acute-virulence factors, and through its cognate response regulator AlgB. However, we show that rather than phosphorylating AlgB, KinB's primary role in the regulation of virulence is to act as a phosphatase to dephosphorylate AlgB and alleviate phosphorylated AlgB's repression of acute virulence.

Links

PubMed PMC3497526 Online version:10.1128/JB.01168-12

Keywords

Gene Expression Regulation, Bacterial; Phosphoric Monoester Hydrolases/metabolism; Phosphorylation; Pseudomonas aeruginosa/enzymology; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/pathogenicity; Virulence; Virulence Factors/biosynthesis

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:Q9HT87

enables

GO:0016791: phosphatase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:ALGB

involved_in

GO:0045862: positive regulation of proteolysis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:ALGB

involved_in

GO:1900378: positive regulation of secondary metabolite biosynthetic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

PSEAE:ALGB

involved_in

GO:2000147: positive regulation of cell motility

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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