PMID:23022132

Citation	Petrezselyova, S, Kinclova-Zimmermannova, O and Sychrova, H' (2012) Vhc1, a novel transporter belonging to the family of electroneutral cation-Cl(-) cotransporters, participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles. Biochim. Biophys. Acta '
Abstract	Cation-Cl(-) cotransporters (CCCs) are integral membrane proteins which catalyze the coordinated symport of Cl(-) with Na(+) and/or K(+) ions in plant and mammalian cells. Here we describe the first Saccharomyces cerevisiae CCC protein, encoded by the YBR235w open reading frame. Subcellular localization studies showed that this yeast CCC is targeted to the vacuolar membrane. Deletion of the YBR235w gene in a salt-sensitive strain (lacking the plasma-membrane cation exporters) resulted in an increased sensitivity to high KCl, altered vacuolar morphology control and decreased survival upon hyperosmotic shock. In addition, deletion of the YBR235w gene in a mutant strain deficient in K(+) uptake produced a significant growth advantage over the parental strain under K(+)-limiting conditions, and a hypersensitivity to the exogenous K(+)/H(+) exchanger nigericin. These results strongly suggest that we have identified a novel yeast vacuolar ion transporter mediating a K(+)-Cl(-) cotransport and playing a role in vacuolar osmoregulation. Considering its identified function, we propose to refer to the yeast YBR235w gene as VHC1 (vacuolar protein homologous to CCC family 1).
Links	PubMed Online version:10.1016/j.bbamem.2012.09.019
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:VHC1	located_in	GO:0031166: integral component of vacuolar membrane	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
YEAST:VHC1	involved_in	GO:0055075: potassium ion homeostasis	ECO:0000316: genetic interaction evidence used in manual assertion	SGD:S000002447 SGD:S000004128	P		Seeded From UniProt	complete
YEAST:VHC1	enables	GO:0015379: potassium:chloride symporter activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
YEAST:VHC1	involved_in	GO:0034486: vacuolar transmembrane transport	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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