GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22987884

From GONUTS
Jump to: navigation, search
Citation

Paparelli, E, Gonzali, S, Parlanti, S, Novi, G, Giorgi, FM, Licausi, F, Kosmacz, M, Feil, R, Lunn, J, Brust, H, van Dongen, J, Steup, M and Perata, P' (2012) Misexpression of a chloroplast aspartyl protease leads to severe growth defects and alters carbohydrate metabolism in Arabidopsis. Plant Physiol. '

Abstract

The crucial role of carbohydrate in plant growth and morphogenesis is widely recognized. In this study we describe the characterization of nana, a dwarf Arabidopsis thaliana mutant impaired in carbohydrate metabolism. We show that the nana dwarf phenotype was accompanied by altered leaf morphology and a delayed flowering time. Our genetic and molecular data indicate that the mutation in nana is due to a T-DNA insertion in the promoter region of a gene encoding a chloroplast-located aspartyl protease that alters its pattern of expression. Overexpression of the gene (oxNANA) phenocopies the mutation. Both nana and oxNANA display alterations in carbohydrate content, and the extent of these changes varies depending on growth light intensity. In particular, in low light soluble sugars levels are lower and do not show the daily fluctuations observed in wild type plants. Moreover, nana and oxNANA are defective in expression of some genes implicated in sugar metabolism and photosynthetic light harvesting. Interestingly, some chloroplast-encoded genes as well as genes, whose products seem to be involved in the retrograde signaling, appear to be down-regulated. These findings suggest that the NANA aspartic protease has an important regulatory function in chloroplasts that not only influences photosynthetic carbon metabolism but also plastid and nuclear gene expression.

Links

PubMed Online version:10.1104/pp.112.204016

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:NANA

involved_in

GO:0007623: circadian rhythm

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NANA

involved_in

GO:0009744: response to sucrose

ECO:0000270: expression pattern evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NANA

located_in

GO:0009507: chloroplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:NANA

acts_upstream_of_or_within

GO:0005975: carbohydrate metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NANA

acts_upstream_of_or_within

GO:0010019: chloroplast-nucleus signaling pathway

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:NANA

enables

GO:0004190: aspartic-type endopeptidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.