GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22922822

From GONUTS
Jump to: navigation, search
Citation

Bond, SR, Wang, N, Leybaert, L and Naus, CC (2012) Pannexin 1 ohnologs in the teleost lineage. J. Membr. Biol. 245:483-93

Abstract

Advances in genomic analysis indicate that the early chordate lineage underwent two whole-genome duplication events in fairly rapid succession around 400-600 million years ago, and that a third duplication event punctuated the radiation of ray-finned fishes (teleosts) around 320-350 million years ago. Connexin ohnologs have been disproportionately well maintained in the teleost genome following this third event, implying that gap junction proteins are amenable to neofunctionalization. A second family of gap junction-like proteins, the pannexins, is also present in chordates, but expansion of this family following the teleost whole-genome duplication has not been addressed in the literature. In the current study we report that ohnologs of panx1 are expressed by zebrafish, and orthologs of these two genes can be found in various other teleost species. The genomic locality of each gene is described, along with sequence alignments that reveal conservation of classic pannexin-specific features/motifs. The transcripts were then cloned from cDNA for in vitro analysis, and both are shown to traffic to the plasma membrane when exogenously expressed. Furthermore, electrophysiological recordings show differences in the biophysical properties between the channels formed by these two proteins. Our results indicate that both copies of the ancestral teleost panx1 gene were conserved following the last whole-genome duplication event and, following conventional zebrafish nomenclature, should now be referred to as panx1a and panx1b.

Links

PubMed Online version:10.1007/s00232-012-9497-4

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DANRE:A0A2R8Q2M0

enables

GO:0022829: wide pore channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:A0A2R8Q2M0

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:A0A2R8Q2M0

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:B1P2E7

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:B1P2E8

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:B1P2E9

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1QKJ1

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1QSR7

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1QSR7

enables

GO:0022829: wide pore channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:F1QSR7

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1QU79

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1R791

enables

GO:0022829: wide pore channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:F1R791

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:F1R791

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:Q1LUA3

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:Q7ZUN0

enables

GO:0022829: wide pore channel activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:Q7ZUN0

located_in

GO:0032587: ruffle membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:Q7ZUN0

located_in

GO:0005886: plasma membrane

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:S4TYN0

located_in

GO:0031410: cytoplasmic vesicle

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.