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PMID:22916179

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Citation

Neumetzler, L, Humphrey, T, Lumba, S, Snyder, S, Yeats, TH, Usadel, B, Vasilevski, A, Patel, J, Rose, JK, Persson, S and Bonetta, D (2012) The FRIABLE1 Gene Product Affects Cell Adhesion in Arabidopsis. PLoS ONE 7:e42914

Abstract

Cell adhesion in plants is mediated predominantly by pectins, a group of complex cell wall associated polysaccharides. An Arabidopsis mutant, friable1 (frb1), was identified through a screen of T-DNA insertion lines that exhibited defective cell adhesion. Interestingly, the frb1 plants displayed both cell and organ dissociations and also ectopic defects in organ separation. The FRB1 gene encodes a Golgi-localized, plant specific protein with only weak sequence similarities to known proteins (DUF246). Unlike other cell adhesion deficient mutants, frb1 mutants do not have reduced levels of adhesion related cell wall polymers, such as pectins. Instead, FRB1 affects the abundance of galactose- and arabinose-containing oligosaccharides in the Golgi. Furthermore, frb1 mutants displayed alteration in pectin methylesterification, cell wall associated extensins and xyloglucan microstructure. We propose that abnormal FRB1 action has pleiotropic consequences on wall architecture, affecting both the extensin and pectin matrices, with consequent changes to the biomechanical properties of the wall and middle lamella, thereby influencing cell-cell adhesion.

Links

PubMed PMC3419242 Online version:10.1371/journal.pone.0042914

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ARATH:FRB1

involved_in

GO:0007155: cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Q9LFC6

GO:0005794: Golgi apparatus

ECO:0000314:

C

Figure 5 shows GFP marked Frb1 colocalizes with a golgi marker.

complete
CACAO 5371

ARATH:Q9LFC6

GO:0080157: regulation of plant-type cell wall organization or biogenesis

ECO:0000315:

P

Figure 7 shows upregulation in cell wall genes in plants that have mutant Frb1

complete
CACAO 5372

ARATH:Q9LFC6

part_of

GO:0005794: Golgi apparatus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

ARATH:Q9LFC6

involved_in

GO:0080157: regulation of plant-type cell wall organization or biogenesis

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

ARATH:Q9LFC6

involved_in

GO:0007155: cell adhesion

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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