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PMID:22902557

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Citation

Olivieri, D, Senti, KA, Subramanian, S, Sachidanandam, R and Brennecke, J (2012) The cochaperone shutdown defines a group of biogenesis factors essential for all piRNA populations in Drosophila. Mol. Cell 47:954-69

Abstract

In animal gonads, PIWI proteins and their bound 23-30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches of piRNA biogenesis, namely primary processing and ping-pong amplification, have been proposed. Despite an overall conceptual understanding of piRNA biogenesis, identity and/or function of the involved players are largely unknown. Here, we demonstrate an essential role for the female sterility gene shutdown in piRNA biology. Shutdown, an evolutionarily conserved cochaperone collaborates with Hsp90 during piRNA biogenesis, potentially at the loading step of RNAs into PIWI proteins. We demonstrate that Shutdown is essential for both primary and secondary piRNA populations in Drosophila. An extension of our study to previously described piRNA pathway members revealed three distinct groups of biogenesis factors. Together with data on how PIWI proteins are wired into primary and secondary processing, we propose a unified model for piRNA biogenesis.

Links

PubMed PMC3463805 Online version:10.1016/j.molcel.2012.07.021

Keywords

Animals; Argonaute Proteins/metabolism; Cells, Cultured; DNA Transposable Elements; Drosophila Proteins/genetics; Drosophila Proteins/metabolism; Drosophila melanogaster/genetics; Drosophila melanogaster/metabolism; HSP90 Heat-Shock Proteins/metabolism; Molecular Chaperones/metabolism; RNA Interference; RNA, Small Interfering/genetics; RNA, Small Interfering/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

DROME:AUB

located_in

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:HSP83

located_in

GO:0048471: perinuclear region of cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:PIWI

located_in

GO:0005634: nucleus

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:FKBP6

located_in

GO:0070725: Yb body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:FKBP6

located_in

GO:0043186: P granule

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DROME:FKBP6

involved_in

GO:0034587: piRNA metabolic process

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DROME:AUB

part_of

GO:0005737: cytoplasm

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

Notes

See also

References

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