GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:22878415

From GONUTS
Jump to: navigation, search
Citation

Schulz, S, Chachami, G, Kozaczkiewicz, L, Winter, U, Stankovic-Valentin, N, Haas, P, Hofmann, K, Urlaub, H, Ovaa, H, Wittbrodt, J, Meulmeester, E and Melchior, F (2012) Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO Rep. 13:930-8

Abstract

Isopeptidases are essential regulators of protein ubiquitination and sumoylation. However, only two families of SUMO isopeptidases are at present known. Here, we report an activity-based search with the suicide inhibitor haemagglutinin (HA)-SUMO-vinylmethylester that led to the identification of a surprising new SUMO protease, ubiquitin-specific protease-like 1 (USPL1). Indeed, USPL1 neither binds nor cleaves ubiquitin, but is a potent SUMO isopeptidase both in vitro and in cells. C13orf22l--an essential but distant zebrafish homologue of USPL1--also acts on SUMO, indicating functional conservation. We have identified invariant USPL1 residues required for SUMO binding and cleavage. USPL1 is a low-abundance protein that colocalizes with coilin in Cajal bodies. Its depletion does not affect global sumoylation, but causes striking coilin mislocalization and impairs cell proliferation, functions that are not dependent on USPL1 catalytic activity. Thus, USPL1 represents a third type of SUMO protease, with essential functions in Cajal body biology.

Links

PubMed PMC3463963 Online version:10.1038/embor.2012.125

Keywords


Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HUMAN:SUMO3

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5W0Q7

F

Seeded From UniProt

complete

HUMAN:SUMO2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5W0Q7

F

Seeded From UniProt

complete

HUMAN:SUMO2

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q6DRC5

F

Seeded From UniProt

complete

HUMAN:SUMO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q5W0Q7

F

Seeded From UniProt

complete

HUMAN:SUMO1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:Q9P0U3

F

Seeded From UniProt

complete

HUMAN:USPL1

enables

GO:0016929: deSUMOylase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:USPL1

involved_in

GO:0016926: protein desumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:USPL1

located_in

GO:0015030: Cajal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

HUMAN:USPL1

NOT|enables

GO:0043130: ubiquitin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:USPL1

enables

GO:0032183: SUMO binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HUMAN:USPL1

involved_in

GO:0008283: cell population proliferation

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:USPL1

involved_in

GO:0030576: Cajal body organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

HUMAN:USPL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P55854

F

Seeded From UniProt

complete

HUMAN:USPL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P61956

F

Seeded From UniProt

complete

HUMAN:USPL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P63165

F

Seeded From UniProt

complete

DANRE:USPL1

enables

GO:0016929: deSUMOylase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:USPL1

involved_in

GO:0016926: protein desumoylation

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:USPL1

located_in

GO:0015030: Cajal body

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

DANRE:USPL1

NOT|enables

GO:0043130: ubiquitin binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:USPL1

enables

GO:0032183: SUMO binding

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

DANRE:USPL1

involved_in

GO:0030576: Cajal body organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

DANRE:USPL1

involved_in

GO:1904867: protein localization to Cajal body

ECO:0000315: mutant phenotype evidence used in manual assertion

ZFIN:ZDB-GENO-121010-1

P

Seeded From UniProt

complete

DANRE:USPL1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P61956

F

Seeded From UniProt

complete

HUMAN:SENP1

enables

GO:0005515: protein binding

ECO:0000353: physical interaction evidence used in manual assertion

UniProtKB:P63165

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.