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PMID:22878415
| Citation |
Schulz, S, Chachami, G, Kozaczkiewicz, L, Winter, U, Stankovic-Valentin, N, Haas, P, Hofmann, K, Urlaub, H, Ovaa, H, Wittbrodt, J, Meulmeester, E and Melchior, F (2012) Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO Rep. 13:930-8 |
|---|---|
| Abstract |
Isopeptidases are essential regulators of protein ubiquitination and sumoylation. However, only two families of SUMO isopeptidases are at present known. Here, we report an activity-based search with the suicide inhibitor haemagglutinin (HA)-SUMO-vinylmethylester that led to the identification of a surprising new SUMO protease, ubiquitin-specific protease-like 1 (USPL1). Indeed, USPL1 neither binds nor cleaves ubiquitin, but is a potent SUMO isopeptidase both in vitro and in cells. C13orf22l--an essential but distant zebrafish homologue of USPL1--also acts on SUMO, indicating functional conservation. We have identified invariant USPL1 residues required for SUMO binding and cleavage. USPL1 is a low-abundance protein that colocalizes with coilin in Cajal bodies. Its depletion does not affect global sumoylation, but causes striking coilin mislocalization and impairs cell proliferation, functions that are not dependent on USPL1 catalytic activity. Thus, USPL1 represents a third type of SUMO protease, with essential functions in Cajal body biology. |
| Links |
PubMed PMC3463963 Online version:10.1038/embor.2012.125 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
HUMAN:SUMO3 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q5W0Q7 |
F |
Seeded From UniProt |
complete | |
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q5W0Q7 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q6DRC5 |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:SUMO1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q5W0Q7 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:SUMO1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:Q9P0U3 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:USPL1 |
enables |
GO:0016929: deSUMOylase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
involved_in |
GO:0016926: protein desumoylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
located_in |
GO:0015030: Cajal body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
NOT|enables |
GO:0043130: ubiquitin binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
enables |
GO:0032183: SUMO binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
involved_in |
GO:0008283: cell population proliferation |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
involved_in |
GO:0030576: Cajal body organization |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
HUMAN:USPL1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P55854 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:USPL1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P61956 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:USPL1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P63165 |
F |
Seeded From UniProt |
complete | |
|
DANRE:USPL1 |
enables |
GO:0016929: deSUMOylase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
involved_in |
GO:0016926: protein desumoylation |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
located_in |
GO:0015030: Cajal body |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
NOT|enables |
GO:0043130: ubiquitin binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
enables |
GO:0032183: SUMO binding |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
involved_in |
GO:0030576: Cajal body organization |
ECO:0000315: mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | ||
|
DANRE:USPL1 |
involved_in |
GO:1904867: protein localization to Cajal body |
ECO:0000315: mutant phenotype evidence used in manual assertion |
ZFIN:ZDB-GENO-121010-1 |
P |
Seeded From UniProt |
complete | |
|
DANRE:USPL1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P61956 |
F |
Seeded From UniProt |
complete | |
|
HUMAN:SENP1 |
enables |
GO:0005515: protein binding |
ECO:0000353: physical interaction evidence used in manual assertion |
UniProtKB:P63165 |
F |
Seeded From UniProt |
complete | |
See also
References
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